Effect of sesamin on apoptosis and cell cycle arrest in human breast cancer MCF-7 cells

An Ci Siao, Chien Wei Hou, Yung Hsi Kao, Kee Ching Jeng

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Dietary prevention has been known to reduce breast cancer risk. Sesamin is one of the major components in sesame seeds and has been widely studied and proven to have anti-proliferation and anti-angiogenic effects on cancer cells. In this study, the influence of sesamin was tested in the human breast cancer MCF-7 cell line for cell viability (MTT assay) and cell cycling (flow cytometry). Results showed that sesamin dose-dependently (1, 10 and 50 μM) reduced the cell viability and increased LDH release and apoptosis (TUNEL assay). In addition, there was a significant increase of sub-G1 phase arrest in the cell cycle after sesamin treatment. Furthermore, sesamin increased the expression of apoptotic markers of Bax, caspase-3, and cell cycle control proteins, p53 and checkpoint kinase 2. Taken together, these results suggested that sesamin might be used as a dietary supplement f or prevention of breast cancer by modulating apoptotic signal pathways and inhibiting tumor cell growth.

Original languageEnglish
Pages (from-to)3779-3783
Number of pages5
JournalAsian Pacific Journal of Cancer Prevention
Volume16
Issue number9
DOIs
StatePublished - 2015

Keywords

  • Apoptosis
  • Breast cancer
  • Cell cycle
  • Sesamin

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