Direct detection of DNA using electrical double layer gated high electron mobility transistor in high ionic strength solution with high sensitivity and specificity

Yen Wen Chen, Tse Yu Tai, Chen Pin Hsu, Indu Sarangadharan, Anil Kumar Pulikkathodi, Hsin Li Wang, Revathi Sukesan, Geng Yen Lee, Jen Inn Chyi, Chih Chen Chen, Gwo Bin Lee, Yu Lin Wang

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

In this research, we have realized an electrical double layer (EDL) gated high electron mobility transistor (HEMT) as DNA sensor. The sensing area on the gate electrode which is separated from the transistor channel is immobilized with probe DNA to capture target DNA from physiological salt environment. The detection limit of the sensor can be as low as 1 fM with very high sensitivity. The specificity of the DNA sensor is also demonstrated by controlling the hybridization temperature. By choosing the hybridization temperature slightly lower than the melting temperature of the well-matched sequence, the binding ratio can be controlled between the fully-matched and mismatched one. The sensor has demonstrated specificity, with the ability to achieve single base mismatch resolution. The sensor has the potential for rapid DNA sensing applications in cells, biomarkers and viruses.

Original languageEnglish
Pages (from-to)110-117
Number of pages8
JournalSensors and Actuators, B: Chemical
Volume271
DOIs
StatePublished - 15 Oct 2018

Keywords

  • AlGaN/GaN HEMT
  • Electrical double layer
  • Sensitivity and specificity of DNA detection

Fingerprint

Dive into the research topics of 'Direct detection of DNA using electrical double layer gated high electron mobility transistor in high ionic strength solution with high sensitivity and specificity'. Together they form a unique fingerprint.

Cite this