Development of lipid targeting Raman probes for in vivo imaging of Caenorhabditis elegans

Shobhit Charan, Fan Ching Chien, Narendra Singh, Chiung Wen Kuo, Peilin Chen

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

A simple, sensitive, and highly specific lipid targeting Raman probe (Nile red coated silver nanoparticles) has been developed to image living nematode Caenorhabditis elegans (C. elegans). Our idea of imaging lipids in C. elegans is to combine the specificity of the fluorescent dye, Nile red, and the highly enhanced Raman scattering on the silver nanoparticles. Our strategy involves the fabrication of a lipid targeting probe, which is incorporated into the intracellular intestinal granules of C. elegans by incubating these worms in the solution containing Raman probes, resulting in an uptake and subsequent incorporation of these Raman probes into the intestinal granule, thus allowing fast visualization of lipid droplets through a conventional confocal imaging technique.

Original languageEnglish
Pages (from-to)5165-5170
Number of pages6
JournalChemistry - A European Journal
Volume17
Issue number18
DOIs
StatePublished - 26 Apr 2011

Keywords

  • Raman probes
  • SERS imaging
  • fluorescence
  • lipids
  • nanoparticles

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