Determination of chronological aging parameters in epidermal keratinocytes by in vivo harmonic generation microscopy

Yi Hua Liao, Szu Yu Chen, Sin Yo Chou, Pei Hsun Wang, Ming Rung Tsai, Chi Kuang Sun

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Skin aging is an important issue in geriatric and cosmetic dermatology. To quantitatively analyze changes in keratinocytes related to intrinsic aging, we exploited a 1230 nm-based in vivo harmonic generation microscopy, combining second- and third-harmonic generation modalities. 52 individuals (21 men and 31 women, age range 19-79) were examined on the sun-protected volar forearm. Through quantitative analysis by the standard algorithm provided, we found that the cellular and nuclear size of basal keratinocytes, but not that of granular cells, was significantly increased with advancing age. The cellular and nuclear areas, which have an increase of 0.51 μm2 and 0.15 μm2 per year, respectively, can serve as scoring indices for intrinsic skin aging.

Original languageEnglish
Pages (from-to)77-88
Number of pages12
JournalBiomedical Optics Express
Volume4
Issue number1
DOIs
StatePublished - 2013

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