Determination of alanine aminotransferase with an electrochemical nano Ir-C biosensor for the screening of liver diseases

Chang Jung Hsueh, Joanne H. Wang, Liming Dai, Chung Chiun Liu

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Alanine aminotransaminase (ALT), is an enzyme that normally resides in serum and body tissues, especially in the liver. It is released into the serum as a result of tissue injury; hence the concentration of ALT in the serum may be increased with acute damage to hepatic cells. A single use, disposable biosensor, comprising iridium nano-particle as catalyst dispersed on carbon paste, has been developed for the determination of ALT concentration. The biosensor is based on quantifying H2O2 concentration produced by a serial of ALT enzymatic reactions. It operates well at room temperature in different physiological fluids: phosphate buffer, calf serum and human serum for ALT concentration of 0-544 ng/mL. Experimental results in human serum are compared to those obtained by spectrophotometric assays with excellent agreement. Therefore, the Ir/C biosensor shows good relationship on the dilution of concentrated ALT clinical applications.

Original languageEnglish
Pages (from-to)107-117
Number of pages11
JournalBiosensors
Volume1
Issue number3
DOIs
StatePublished - Sep 2011

Keywords

  • Alanine Aminotransferase
  • Biosensor
  • Electrochemical Detection
  • Liver Diseases

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