TY - JOUR
T1 - Control of dengue and Zika virus vector Aedes aegypti using the predatory copepod Megacyclops formosanus
T2 - Synergy with Hedychium coronarium-synthesized silver nanoparticles and related histological changes in targeted mosquitoes
AU - Kalimuthu, Kandasamy
AU - Panneerselvam, Chellasamy
AU - Chou, Chi
AU - Tseng, Li Chun
AU - Murugan, Kadarkarai
AU - Tsai, Kun Hsien
AU - Alarfaj, Abdullah A.
AU - Higuchi, Akon
AU - Canale, Angelo
AU - Hwang, Jiang Shiou
AU - Benelli, Giovanni
N1 - Publisher Copyright:
© 2017 Institution of Chemical Engineers
PY - 2017
Y1 - 2017
N2 - The employ of nano-formulated biopesticides for mosquito control represents a promising strategy. Recently, it has been outlined that differences in the green reducing extracts used in nanosynthesis led to the production of nanoparticles with distinct bio-physical properities. In the present investigation, Hedychium coronarium-synthesized silver nanoparticles (AgNPs) were characterized by UV–vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), energy-dispersive X-ray diffraction (EDX), and transmission electron microscopy (TEM). The toxicity of H. coronarium rhizome extract and H. coronarium-synthesized AgNPs was assessed against larvae and pupae of the dengue vector Aedes aegypti, as well as against adults of the non-target copepod Mesocyclops formosanus. Then, the control of A. aegypti larval population was attempted using the predatory copepod M. formosanus in synergy with H. coronarium-synthesized silver nanoparticles, evaluating predation efficiency pre- and post-nanoparticle treatment. In presence of both predaceous copepods and nano-larvicides high control of the larval populations was obtained. Furthermore, histological changes in mosquitoes targeted with nanopesticides were studied by light microscopy, evaluating the impact on mid-gut epithelial cells following treatment with LC50 of AgNP. Overall, this study highlighted the concrete potential of synergizing copepod-based control programs with highly effective green nano-larvicides in the fight against dengue and Zika virus vectors.
AB - The employ of nano-formulated biopesticides for mosquito control represents a promising strategy. Recently, it has been outlined that differences in the green reducing extracts used in nanosynthesis led to the production of nanoparticles with distinct bio-physical properities. In the present investigation, Hedychium coronarium-synthesized silver nanoparticles (AgNPs) were characterized by UV–vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), energy-dispersive X-ray diffraction (EDX), and transmission electron microscopy (TEM). The toxicity of H. coronarium rhizome extract and H. coronarium-synthesized AgNPs was assessed against larvae and pupae of the dengue vector Aedes aegypti, as well as against adults of the non-target copepod Mesocyclops formosanus. Then, the control of A. aegypti larval population was attempted using the predatory copepod M. formosanus in synergy with H. coronarium-synthesized silver nanoparticles, evaluating predation efficiency pre- and post-nanoparticle treatment. In presence of both predaceous copepods and nano-larvicides high control of the larval populations was obtained. Furthermore, histological changes in mosquitoes targeted with nanopesticides were studied by light microscopy, evaluating the impact on mid-gut epithelial cells following treatment with LC50 of AgNP. Overall, this study highlighted the concrete potential of synergizing copepod-based control programs with highly effective green nano-larvicides in the fight against dengue and Zika virus vectors.
KW - Biological control
KW - Biosafety
KW - Biosynthesis
KW - Dengue
KW - Nanosynthesis
KW - Zika virus
UR - http://www.scopus.com/inward/record.url?scp=85017403339&partnerID=8YFLogxK
U2 - 10.1016/j.psep.2017.03.027
DO - 10.1016/j.psep.2017.03.027
M3 - 期刊論文
AN - SCOPUS:85017403339
SN - 0957-5820
VL - 109
SP - 82
EP - 96
JO - Process Safety and Environmental Protection
JF - Process Safety and Environmental Protection
ER -
