Control of dengue and Zika virus vector Aedes aegypti using the predatory copepod Megacyclops formosanus: Synergy with Hedychium coronarium-synthesized silver nanoparticles and related histological changes in targeted mosquitoes

Kandasamy Kalimuthu, Chellasamy Panneerselvam, Chi Chou, Li Chun Tseng, Kadarkarai Murugan, Kun Hsien Tsai, Abdullah A. Alarfaj, Akon Higuchi, Angelo Canale, Jiang Shiou Hwang, Giovanni Benelli

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

The employ of nano-formulated biopesticides for mosquito control represents a promising strategy. Recently, it has been outlined that differences in the green reducing extracts used in nanosynthesis led to the production of nanoparticles with distinct bio-physical properities. In the present investigation, Hedychium coronarium-synthesized silver nanoparticles (AgNPs) were characterized by UV–vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), energy-dispersive X-ray diffraction (EDX), and transmission electron microscopy (TEM). The toxicity of H. coronarium rhizome extract and H. coronarium-synthesized AgNPs was assessed against larvae and pupae of the dengue vector Aedes aegypti, as well as against adults of the non-target copepod Mesocyclops formosanus. Then, the control of A. aegypti larval population was attempted using the predatory copepod M. formosanus in synergy with H. coronarium-synthesized silver nanoparticles, evaluating predation efficiency pre- and post-nanoparticle treatment. In presence of both predaceous copepods and nano-larvicides high control of the larval populations was obtained. Furthermore, histological changes in mosquitoes targeted with nanopesticides were studied by light microscopy, evaluating the impact on mid-gut epithelial cells following treatment with LC50 of AgNP. Overall, this study highlighted the concrete potential of synergizing copepod-based control programs with highly effective green nano-larvicides in the fight against dengue and Zika virus vectors.

Original languageEnglish
Pages (from-to)82-96
Number of pages15
JournalProcess Safety and Environmental Protection
Volume109
DOIs
StatePublished - 2017

Keywords

  • Biological control
  • Biosafety
  • Biosynthesis
  • Dengue
  • Nanosynthesis
  • Zika virus

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