Comparison of Escherichia coli surface attachment methods for single-cell microscopy

Yao Kuan Wang, Ekaterina Krasnopeeva, Ssu Yuan Lin, Fan Bai, Teuta Pilizota, Chien Jung Lo

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

For in vivo, single-cell imaging bacterial cells are commonly immobilised via physical confinement or surface attachment. Different surface attachment methods have been used both for atomic force and optical microscopy (including super resolution), and some have been reported to affect bacterial physiology. However, a systematic comparison of the effects these attachment methods have on the bacterial physiology is lacking. Here we present such a comparison for bacterium Escherichia coli, and assess the growth rate, size and intracellular pH of cells growing attached to different, commonly used, surfaces. We demonstrate that E. coli grow at the same rate, length and internal pH on all the tested surfaces when in the same growth medium. The result suggests that tested attachment methods can be used interchangeably when studying E. coli physiology.

Original languageEnglish
Article number19418
JournalScientific Reports
Volume9
Issue number1
DOIs
StatePublished - 1 Dec 2019

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