Chitosan/PVA Hetero-Composite Hydrogel Containing Antimicrobials, Perfluorocarbon Nanoemulsions, and Growth Factor-Loaded Nanoparticles as a Multifunctional Dressing for Diabetic Wound Healing: Synthesis, Characterization, and In Vitro/In Vivo Evaluation

Yu Hsiang Lee, Sheng Jhe Lin

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Diabetic foot ulcers remain one of the most difficult-to-treat complications of diabetes and may seriously threaten the life of patients since it frequently results in limb loss due to amputation, suggesting that an effective therapeutic strategy is still urgently needed. In this study, a chitosan-based heterogeneous composite hydrogel encapsulating perfluorocarbon emulsions, epidermal growth factor (EGF)-loaded chitosan nanoparticles, and polyhexamethylene biguanide (PHMB) named PE ENP PCH was developed for diabetic wound healing. The PE ENP PCH could sustainably release EGF and PHMB in an ion-rich environment to exert antibacterial effects and promote cell growth for wound repair. In addition, the PE ENP PCH can provide anti-inflammatory effects functioned by its main constituent of chitosan. Moreover, the PE ENP PCH can proactively offer oxygen delivery through the incorporation of perfluorocarbon and, therefore, is able to alleviate hypoxia conditions on diabetic wounds. These functionalities enabled a markedly enhanced wound healing efficacy on diabetic rats treated with the PE ENP PCHs, including thorough re-epithelization, a reduced inflammatory response, faster collagen deposition, and advanced collagen maturation resulting in a 95% of wound closure degree after 15 days that was 12.6% (p < 0.05) higher than the value of the group treated with the commercial dressing HeraDerm. Given the aforementioned advantages, together with the known merits of hydrogels, the developed PE ENP PCH is anticipated to be a feasible tool for clinical diabetic wound treatment.

Original languageEnglish
Article number537
JournalPharmaceutics
Volume14
Issue number3
DOIs
StatePublished - Mar 2022

Keywords

  • Anti-inflammatory
  • Antibacterial
  • Cell growth
  • Diabetic wound healing
  • Hydrogel
  • Nanoparticle
  • Oxygen delivery
  • Perfluorocarbon

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