Cell separation and culture of endothelial cells through nylon net filter from human peripheral blood

Yoshimi Sakashita, Masahiro Noda, Yumiko Gomei, Satsuki Egashira, Shizue Natori, Akon Higuchi

Research output: Contribution to conferencePaperpeer-review

Abstract

Blood cell separation is one of the important technologies in transplantation of blood cells and endothelial cells to patients. Membrane filtration has been used for the industrial separation of blood cells. Membrane filtration technology has merits of easy and simple operation, sanitary aspects and an inexpensive method compared to other cell separation methods. However, only few studies have been reported for the blood cell separation using membrane filtration. Here we report the cell separation between blood cells and endothelial cells through Nylon net filter. Human Umbilical Vascular Endothelial Cells (HUVEC) in recovery solution was purified by centrifugation and was cultivated for 7 days, after isolation of HUVEC through Nylon net filters from peripheral blood.

Original languageEnglish
Pages1919
Number of pages1
StatePublished - 2006
Event55th SPSJ Annual Meeting - Nagoya, Japan
Duration: 24 May 200626 May 2006

Conference

Conference55th SPSJ Annual Meeting
Country/TerritoryJapan
CityNagoya
Period24/05/0626/05/06

Keywords

  • Cell Culture
  • Cell separation
  • Endothelial Cell
  • Membrane

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