Cell lysis by low power focused acoustic transducer and investigation of acoustic intensity threshold for cytolysis of various cell lines

Lingtao Wang, Yi Jia Li, Anderson Lin, Shih Jui Chen, Mitchell Gross, Eun Sok Kim

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

This paper reports recent development of micron-sized cell lysis without noticeable acoustic cavitation by a Self Focused Acoustic Transducer (SFAT). Localized cell lysis was obtained with prostate cancer cell lines and breast cancer cell lines by applying various power intensity levels at the transducer's fundamental and 3rd harmonic resonant frequencies (17.3 and 56 MHz, respectively). The cell lysis area was around 100 μm (fundamental) and 50 μm (third harmonic) in diameter. According to the results, the cell lysis area depended on the acoustic-wave frequency, but not on the applied acoustic intensity above the threshold intensity. Also, different cell lines showed varied sensitivity to focused acoustic energy, and had different energy threshold for localized cytolysis.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages434-436
Number of pages3
StatePublished - 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: 2 Oct 20116 Oct 2011

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume1

Conference

Conference15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Country/TerritoryUnited States
CitySeattle, WA
Period2/10/116/10/11

Keywords

  • Cancer
  • Localized cytolysis
  • Micro-electro-mechanical-system (MEMS)
  • Self focused acoustic transducer (SFAT)

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