Cell detachment and preservation of hematopoietic stem cells on thermo-responsible pluronicimmobilized membranes

Akon Higuchi, Satsuki Egashira, Yuki Matsuoka

Research output: Contribution to conferencePaperpeer-review

Abstract

Long-term (7 days) cell culture was successively performed on the Pluronic-immobilized flask in the current investigation. The Pluronic F127 and F68, a triblock copolymer of ethylene oxide, and propylene oxide was activated using carbonyldiimidazole (CDI), and CDI-activated Pluronic was subsequently immobilized on the surface of a lysine-coated polystyrene tissue culture flask. L929 cells were successively detached from the Pluronic-immobilized flask by cooling the flask to 4-15°C. This detachment is due to the hydration and dehydration properties of Pluronic F127, depending on the temperature. Furthermore, we show that hematopoietic stem cells in umbilical cord blood are extensively preserved in the Pluronic-immobilized flask under bio-inert conditions compared to those in commercially available tissue culture flask. We anticipate that this is caused by the flexible brush-like segments of Pluronic on the tissue culture flask. The flexible segments of Pluronic induce hematopoietic stem cells to bio-inert cultivation of hematopoietic stem cells.

Original languageEnglish
Pages4957-4958
Number of pages2
StatePublished - 2006
Event55th Society of Polymer Science Japan Symposium on Macromolecules - Toyama, Japan
Duration: 20 Sep 200622 Sep 2006

Conference

Conference55th Society of Polymer Science Japan Symposium on Macromolecules
Country/TerritoryJapan
CityToyama
Period20/09/0622/09/06

Keywords

  • CD34
  • Cell culture
  • Hematopoietic stem cell
  • Pluronic
  • Surface modification

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