Burkholderia pseudomallei infection induces the expression of apoptosis-related genes and proteins in mouse macrophages

You Cheng Hseu, Jia Chuen Sung, Bao Sen Shieh, Ssu C.hing Chen

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

BACKGROUND/PURPOSE: In this study, we addressed whether the production of apoptosis-related genes and proteins is induced in mouse macrophages infected with Burkholderia pseudomallei cells.

METHODS: Mouse macrophages were infected with B. pseudomallei cells at 0.5 hours, 1 hour, 2 hours, 4 hours, and 6 hours, respectively, followed by real-time polymerase chain reaction (PCR) array analysis. The amount of apoptosis-related proteins (caspase-3, caspase -8, caspase -9, Bax, and Bcl-2) was confirmed by Western blot.

RESULTS: After infection, an increase of these proteins was observed. The expression levels of other apoptosis-related genes were also determined by PCR array. Experimental results revealed that the messenger RNA levels of tumor necrosis factor ligand (e.g., tnfsf10 and tnfrs10b) and fas were increased, whereas the expression levels of some antiapoptosis genes such as Birc5, Hells, and Bnip3 were decreased.

CONCLUSION: Our study results demonstrate that the apoptosis-related genes and proteins in mouse macrophages were modulated by B. pseudomallei.

Original languageEnglish
Pages (from-to)394-398
Number of pages5
JournalJournal of Microbiology, Immunology and Infection
Volume47
Issue number5
DOIs
StatePublished - 1 Oct 2014

Keywords

  • Apoptosis-based polymerase chain reaction array
  • Burkholderia pseudomallei
  • Caspases

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