Biomolecular imaging based on far-red fluorescent protein with a high two-photon excitation action cross section

Tsung Han Tsai, Cheng Yung Lin, Huai Jen Tsai, Szu Yu Chen, Shih Peng Tai, Kung Hsuan Lin, Chi Kuang Sun

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

The two-photon excitation action cross section of Hc-Red fluorescent proteins (Hc-RFPs) is measured and found to be of the same order as that of enhanced green fluorescent proteins. With a 618 nm emission wave-length in the far-red region and with an excitation wavelength around 1200 nm, Hc-RPF-based two-photon fluorescence microscopy (2PFM) can offer deep penetration capability inside live samples and is ideal for in vivo gene expression study and biomolecular imaging in live objects. In vivo 2PFM of the developing heart deep inside a transgenic zebrafish embryo tagged by Hc-RFP is also successfully demonstrated.

Original languageEnglish
Pages (from-to)930-932
Number of pages3
JournalOptics Letters
Volume31
Issue number7
DOIs
StatePublished - 1 Apr 2006

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