Biomolecular imaging based on far-red fluorescent protein with a high two-photon excitation action cross section

Tsung Han Tsai; Cheng Yung Lin; Huai Jen Tsai; Szu Yu Chen; Shih Peng Tai; Kung Hsuan Lin; Chi Kuang Sun

doi:10.1364/OL.31.000930

Biomolecular imaging based on far-red fluorescent protein with a high two-photon excitation action cross section

Tsung Han Tsai, Cheng Yung Lin, Huai Jen Tsai, Szu Yu Chen, Shih Peng Tai, Kung Hsuan Lin, Chi Kuang Sun

Department of Optics and Photonics

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

The two-photon excitation action cross section of Hc-Red fluorescent proteins (Hc-RFPs) is measured and found to be of the same order as that of enhanced green fluorescent proteins. With a 618 nm emission wave-length in the far-red region and with an excitation wavelength around 1200 nm, Hc-RPF-based two-photon fluorescence microscopy (2PFM) can offer deep penetration capability inside live samples and is ideal for in vivo gene expression study and biomolecular imaging in live objects. In vivo 2PFM of the developing heart deep inside a transgenic zebrafish embryo tagged by Hc-RFP is also successfully demonstrated.

Original language	English
Pages (from-to)	930-932
Number of pages	3
Journal	Optics Letters
Volume	31
Issue number	7
DOIs	https://doi.org/10.1364/OL.31.000930
State	Published - 1 Apr 2006

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title = "Biomolecular imaging based on far-red fluorescent protein with a high two-photon excitation action cross section",

abstract = "The two-photon excitation action cross section of Hc-Red fluorescent proteins (Hc-RFPs) is measured and found to be of the same order as that of enhanced green fluorescent proteins. With a 618 nm emission wave-length in the far-red region and with an excitation wavelength around 1200 nm, Hc-RPF-based two-photon fluorescence microscopy (2PFM) can offer deep penetration capability inside live samples and is ideal for in vivo gene expression study and biomolecular imaging in live objects. In vivo 2PFM of the developing heart deep inside a transgenic zebrafish embryo tagged by Hc-RFP is also successfully demonstrated.",

author = "Tsai, {Tsung Han} and Lin, {Cheng Yung} and Tsai, {Huai Jen} and Chen, {Szu Yu} and Tai, {Shih Peng} and Lin, {Kung Hsuan} and Sun, {Chi Kuang}",

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T1 - Biomolecular imaging based on far-red fluorescent protein with a high two-photon excitation action cross section

AU - Tsai, Tsung Han

AU - Lin, Cheng Yung

AU - Tsai, Huai Jen

AU - Chen, Szu Yu

AU - Tai, Shih Peng

AU - Lin, Kung Hsuan

AU - Sun, Chi Kuang

PY - 2006/4/1

Y1 - 2006/4/1

N2 - The two-photon excitation action cross section of Hc-Red fluorescent proteins (Hc-RFPs) is measured and found to be of the same order as that of enhanced green fluorescent proteins. With a 618 nm emission wave-length in the far-red region and with an excitation wavelength around 1200 nm, Hc-RPF-based two-photon fluorescence microscopy (2PFM) can offer deep penetration capability inside live samples and is ideal for in vivo gene expression study and biomolecular imaging in live objects. In vivo 2PFM of the developing heart deep inside a transgenic zebrafish embryo tagged by Hc-RFP is also successfully demonstrated.

AB - The two-photon excitation action cross section of Hc-Red fluorescent proteins (Hc-RFPs) is measured and found to be of the same order as that of enhanced green fluorescent proteins. With a 618 nm emission wave-length in the far-red region and with an excitation wavelength around 1200 nm, Hc-RPF-based two-photon fluorescence microscopy (2PFM) can offer deep penetration capability inside live samples and is ideal for in vivo gene expression study and biomolecular imaging in live objects. In vivo 2PFM of the developing heart deep inside a transgenic zebrafish embryo tagged by Hc-RFP is also successfully demonstrated.

UR - http://www.scopus.com/inward/record.url?scp=33646552694&partnerID=8YFLogxK

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DO - 10.1364/OL.31.000930

M3 - 期刊論文

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AN - SCOPUS:33646552694

SN - 0146-9592

VL - 31

SP - 930

EP - 932

JO - Optics Letters

JF - Optics Letters

IS - 7

ER -

Biomolecular imaging based on far-red fluorescent protein with a high two-photon excitation action cross section

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