We assume that adjacent cells on a solid surface may establish a microenvironment with a concentration of an autocrine growth factor (AGF) greater than a threshold value. Cells enclosed in this microenvironment undergo proliferntion as a response to the regulatory action of AGF. If no such microenvironment can be formed, cells remain unchanged on the surface. On the basis of this hypothesis, all the seemingly peculiar behaviors of the cell growth phenomenon under consideration become logical. In particular, the following problems are solved: (i) Why does there exist a critical inoculum density? (ii) Why is the critical inoculum cell number proportional to the radius of a microcarrier with a power less than two? (iii) Why is cell growth on microcarriers appreciable even at a very low level of inoculum density? (iv) Why is the inoculum density requirement for cells growing on petri dishes considerably less than that for cells growing on microcarriers? (v) Why does plating efficiency vary with inoculum density for cells growing on petri dishes, and how does it vary?
|Number of pages||6|
|Journal||Journal of the Chinese Institute of Chemical Engineers|
|State||Published - May 1991|