Arabidopsis HIT1, a putative homolog of yeast tethering protein Vps53p, is required for pollen tube elongation

Lian Chin Wang, Ching Hui Yeh, Ronald J. Sayler, Ya Yun Lee, Chung An Lu, Shaw Jye Wu

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


The Arabidopsis HIT1 gene encodes a protein that is homologous to the yeast tethering factor Vps53p, which is involved in retrograde vesicle trafficking from the endosome to the trans-Golgi network. Although the ethyl methanesulfonate mutagenized hit1-1 allele can be maintained homozygously, T-DNA insertional hit1-2 and hit1-3 mutants can only be isolated as hemizygous lines. No heterozygous progeny were produced in outcrosses to wild-type plants using pollen from either hit1-2 or hit1-3 heterozygotes. The reciprocal cross using pollen from wild-type plants on either hit1-2 or hit1-3 mutants produced heterozygous and wild-type progeny. In reproductive tissues, HIT1 promoter-driven GUS activity was detected only in mature pollen and elongated pollen tubes. In vitro pollen germination further showed that only half the pollen grains from hit1-2 and hit1-3 heterozygote plants produced normal pollen tubes. In contrast, the pollen tube length of pollen grains from the hit1-1 mutant was reduced compared to that of the wild type. These results suggest that HIT1 may govern a vesicle trafficking event that is required for pollen tube tip growth during male gametogenesis and that disruption of HIT1 results in male specific transmission defect. Moreover, while the hit1-1 mutant is partially functional leading to reduced pollen tube length, hit1-2 and hit1-3 are total-loss-of-function alleles.

Original languageEnglish
Pages (from-to)25-32
Number of pages8
JournalBotanical Studies
Issue number1
StatePublished - Jan 2008


  • Arabidopsis thaliana
  • Pollen tube
  • Tip growth
  • Vesicle tethering factor
  • Yeast Vps53p protein


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