Antrodia camphorata inhibits proliferation of human breast cancer cells in vitro and in vivo

You Cheng Hseu, Ssu Ching Chen, Huang Chi Chen, Jiuun Wang Liao, Hsin Ling Yang

Research output: Contribution to journalArticlepeer-review

66 Scopus citations

Abstract

Antrodia camphorata (A. camphorata) has been shown to induce apoptosis in cultured human breast cancer cells (MDA-MB-231). In this study, we report the effectiveness of the fermented culture broth of A. camphorata in terms of tumor regression as determined using both in vitro cell culture and in vivo athymic nude mice models of breast cancer. We found that the A. camphorata treatment decreased the proliferation of MDA-MB-231 cells by arresting progression through the G1 phase of the cell cycle. This cell cycle blockade was associated with reductions in cyclin D1, cyclin E, CDK4, cyclin A, and proliferating cell nuclear antigen (PCNA), and increased CDK inhibitor p27/KIP and p21/WAF1 in a dose and time-dependent manner. Furthermore, the A. camphorata treatment was effective in delaying tumor incidence in the nude mice inoculated with MDA-MB-231 cells as well as reducing the tumor burden when compared to controls. A. camphorata treatment also inhibited proliferation (cyclin D1 and PCNA) and induced apoptosis (Bcl-2 and TUNEL) when the tumor tissue sections were examined histologically and immunohistochemically. These results suggest that the A. camphorata treatment induced cell cycle arrest and apoptosis of human breast cancer cells both in vitro and in vivo.

Original languageEnglish
Pages (from-to)2680-2688
Number of pages9
JournalFood and Chemical Toxicology
Volume46
Issue number8
DOIs
StatePublished - Aug 2008

Keywords

  • Antrodia Camphorata
  • Apoptosis
  • Breast cancer
  • Cell cycle arrest

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