TY - JOUR
T1 - Analyzing Dehalochip
T2 - A functional DNA microarray for reductive dichlorination in chloroethene-contaminated sites
AU - Lu, Che Wei
AU - Kao, Chih Ming
AU - Yao, Chao Ling
AU - Chen, Ssu Ching
N1 - Publisher Copyright:
© 2024
PY - 2024/12/15
Y1 - 2024/12/15
N2 - Interpreting high-throughput transcriptomic and metagenomic data from non-model microorganisms presents a challenge due to the significant number of genes with unknown functions and sequences. In this study, we applied an innovative microarray, Dehalochip, for detecting the expression of genes in various microorganisms, particularly focusing on genes involved in chloroethene degradation. Our results demonstrated that this approach can effectively identify dechlorination genes, such as 16S rRNA, tceA, bvcA, and vcrA, in Dehalococcoides mccartyi from samples of groundwater contaminated with chloroethene. Noticeably, the sensitivity and specificity of our Dehalochip are comparable to that of quantitative PCR. However, it stands out as a more viable option for in-situ applications due to its greater capacity to infer potential dechlorination genes. Consequently, we believe our dechlorination microarray offers valuable insights into the role of known microorganisms and their associated functional genes in chloroethene-contaminated environments. This contributes to a deeper understanding of the in-situ reductive dechlorination process.
AB - Interpreting high-throughput transcriptomic and metagenomic data from non-model microorganisms presents a challenge due to the significant number of genes with unknown functions and sequences. In this study, we applied an innovative microarray, Dehalochip, for detecting the expression of genes in various microorganisms, particularly focusing on genes involved in chloroethene degradation. Our results demonstrated that this approach can effectively identify dechlorination genes, such as 16S rRNA, tceA, bvcA, and vcrA, in Dehalococcoides mccartyi from samples of groundwater contaminated with chloroethene. Noticeably, the sensitivity and specificity of our Dehalochip are comparable to that of quantitative PCR. However, it stands out as a more viable option for in-situ applications due to its greater capacity to infer potential dechlorination genes. Consequently, we believe our dechlorination microarray offers valuable insights into the role of known microorganisms and their associated functional genes in chloroethene-contaminated environments. This contributes to a deeper understanding of the in-situ reductive dechlorination process.
KW - Bioaugmentation
KW - Biostimulation
KW - Dehalococcoides mccartyi
KW - Microarray
UR - http://www.scopus.com/inward/record.url?scp=85206686556&partnerID=8YFLogxK
U2 - 10.1016/j.envpol.2024.125096
DO - 10.1016/j.envpol.2024.125096
M3 - 期刊論文
C2 - 39389249
AN - SCOPUS:85206686556
SN - 0269-7491
VL - 363
JO - Environmental Pollution
JF - Environmental Pollution
M1 - 125096
ER -