An investigation into the influence of secondary structures on DNA hybridization using surface plasmon resonance biosensing

Fan Ching Chien; Jih Shiou Liu; Huang Ju Su; Li An Kao; Chung Fan Chiou; Wen Yih Chen; Shean Jen Chen

doi:10.1016/j.cplett.2004.09.007

An investigation into the influence of secondary structures on DNA hybridization using surface plasmon resonance biosensing

Fan Ching Chien, Jih Shiou Liu, Huang Ju Su, Li An Kao, Chung Fan Chiou, Wen Yih Chen, Shean Jen Chen

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Abstract

This study utilizes a surface plasmon resonance (SPR) biosensing and a theoretical secondary structure calculation to investigate the influence of secondary structures on the DNA hybridization. It is found that the SPR angular shifts associated with the three pairs of 60mer oligonucleotides with prominent secondary structures are lower than those observed for the two pairs of oligonucleotides with no obvious secondary structures. It is also determined that increasing the DNA hybridization temperature from 35 to 45 °C reduces secondary structure effects. On the hybridization with mixture target oligonucleotides, the SPR results demonstrate that secondary structures interfere significantly.

Original language	English
Pages (from-to)	429-434
Number of pages	6
Journal	Chemical Physics Letters
Volume	397
Issue number	4-6
DOIs	https://doi.org/10.1016/j.cplett.2004.09.007
State	Published - 21 Oct 2004

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title = "An investigation into the influence of secondary structures on DNA hybridization using surface plasmon resonance biosensing",

abstract = "This study utilizes a surface plasmon resonance (SPR) biosensing and a theoretical secondary structure calculation to investigate the influence of secondary structures on the DNA hybridization. It is found that the SPR angular shifts associated with the three pairs of 60mer oligonucleotides with prominent secondary structures are lower than those observed for the two pairs of oligonucleotides with no obvious secondary structures. It is also determined that increasing the DNA hybridization temperature from 35 to 45 °C reduces secondary structure effects. On the hybridization with mixture target oligonucleotides, the SPR results demonstrate that secondary structures interfere significantly.",

author = "Chien, {Fan Ching} and Liu, {Jih Shiou} and Su, {Huang Ju} and Kao, {Li An} and Chiou, {Chung Fan} and Chen, {Wen Yih} and Chen, {Shean Jen}",

note = "Funding Information: The current authors gratefully acknowledge the support provided to this study by the National Science Council, Taiwan, under Grant number NSC 92-2623-7-006-016.",

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T1 - An investigation into the influence of secondary structures on DNA hybridization using surface plasmon resonance biosensing

AU - Chien, Fan Ching

AU - Liu, Jih Shiou

AU - Su, Huang Ju

AU - Kao, Li An

AU - Chiou, Chung Fan

AU - Chen, Wen Yih

AU - Chen, Shean Jen

N1 - Funding Information: The current authors gratefully acknowledge the support provided to this study by the National Science Council, Taiwan, under Grant number NSC 92-2623-7-006-016.

PY - 2004/10/21

Y1 - 2004/10/21

N2 - This study utilizes a surface plasmon resonance (SPR) biosensing and a theoretical secondary structure calculation to investigate the influence of secondary structures on the DNA hybridization. It is found that the SPR angular shifts associated with the three pairs of 60mer oligonucleotides with prominent secondary structures are lower than those observed for the two pairs of oligonucleotides with no obvious secondary structures. It is also determined that increasing the DNA hybridization temperature from 35 to 45 °C reduces secondary structure effects. On the hybridization with mixture target oligonucleotides, the SPR results demonstrate that secondary structures interfere significantly.

AB - This study utilizes a surface plasmon resonance (SPR) biosensing and a theoretical secondary structure calculation to investigate the influence of secondary structures on the DNA hybridization. It is found that the SPR angular shifts associated with the three pairs of 60mer oligonucleotides with prominent secondary structures are lower than those observed for the two pairs of oligonucleotides with no obvious secondary structures. It is also determined that increasing the DNA hybridization temperature from 35 to 45 °C reduces secondary structure effects. On the hybridization with mixture target oligonucleotides, the SPR results demonstrate that secondary structures interfere significantly.

UR - http://www.scopus.com/inward/record.url?scp=7444244358&partnerID=8YFLogxK

U2 - 10.1016/j.cplett.2004.09.007

DO - 10.1016/j.cplett.2004.09.007

M3 - 期刊論文

AN - SCOPUS:7444244358

SN - 0009-2614

VL - 397

SP - 429

EP - 434

JO - Chemical Physics Letters

JF - Chemical Physics Letters

IS - 4-6

ER -

An investigation into the influence of secondary structures on DNA hybridization using surface plasmon resonance biosensing

Abstract

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