An Integrated Multi-Function Heterogeneous Biochemical Circuit for High-Resolution Electrochemistry-Based Genetic Analysis

Yifan Dai, Wei Xu, Rodrigo A. Somoza, Jean F. Welter, Arnold I. Caplan, Chung Chiun Liu

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Modular construction of an autonomous and programmable multi-functional heterogeneous biochemical circuit that can identify, transform, translate, and amplify biological signals into physicochemical signals based on logic design principles can be a powerful means for the development of a variety of biotechnologies. To explore the conceptual validity, we design a CRISPR-array-mediated primer-exchange-reaction-based biochemical circuit cascade, which probes a specific biomolecular input, transform the input into a structurally accessible form for circuit wiring, translate the input information into an arbitrary sequence, and finally amplify the prescribed sequence through autonomous formation of a signaling concatemer. This upstream biochemical circuit is further wired with a downstream electrochemical interface, delivering an integrated bioanalytical platform. We program this platform to directly analyze the genome of SARS-CoV-2 in human cell lysate, demonstrating the capability and the utility of this unique integrated system.

Original languageEnglish
Pages (from-to)20545-20551
Number of pages7
JournalAngewandte Chemie - International Edition
Volume59
Issue number46
DOIs
StatePublished - 9 Nov 2020

Keywords

  • bioanalytical chemistry
  • CRISPR
  • electrochemistry
  • genetic circuits
  • primer exchange reaction

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