Amperometric acetylcholine sensor catalyzed by nickel anode electrode

Shin Lin, Chung Chiun Liu, Tse Chuan Chou

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An amperometric method was using a nickel catalytic electrode in aqueous base solution for detecting acetylcholine (ACh). A sensing mechanism was developed in which ACh was hydrolyzed in base aqueous solution to produce the acetic anion and choline. The alcohol group of choline was oxidized to the corresponding carboxylic acid by Ni(OH)2/NiOOH catalytic system. The amperometric response resulted from the current generated by ACh oxidation in response to step changes in ACh concentration. The potential window of limiting current of ACh anodic oxidation at the Ni interface was determined in NaOH electrolyte. The effect of NaOH electrolyte concentration on sensitivity was also discussed. At the optimum operating condition, the method exhibits a good linear relationship between the response current and the ACh concentration. The response time of the ACh sensing system was 10 s. Scanning electrochemical microscopy (SECM) with platinum micro-tips was used to investigate the diffusion layer thickness of Ni electrode.

Original languageEnglish
Pages (from-to)9-14
Number of pages6
JournalBiosensors and Bioelectronics
Issue number1
StatePublished - 30 Jul 2004


  • Acetylcholine
  • Amperometry
  • Nickel
  • SECM
  • Sensor


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