A two-photon fluorescence probe for cell membrane imaging under temporal-focusing multiphoton excitation microscopy (TFMPEM)

Wei Hsuan Lee; Jian Zong Lai; Yu Hsuan Hsu; Fung Yu Cheng; Ching Lung Luo; Yung Chin Huang; Tzu Chau Lin; Fan Ching Chien

doi:10.1039/d1cc04962c

A two-photon fluorescence probe for cell membrane imaging under temporal-focusing multiphoton excitation microscopy (TFMPEM)

Wei Hsuan Lee, Jian Zong Lai, Yu Hsuan Hsu, Fung Yu Cheng, Ching Lung Luo, Yung Chin Huang, Tzu Chau Lin, Fan Ching Chien

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A small-sized chromophore, BTTA-2OH, manifesting favorable solubility, large two-photon excitation efficiency, and good fluorescence photostability was synthesized to label the membrane of living cells for visualizing the dynamic movement of membrane-related vesicles via a two-photon fluorescence imaging technique based on wavelength-tunable temporal-focusing multiphoton excitation microscopy.

Original language	English
Pages (from-to)	13118-13121
Number of pages	4
Journal	Chemical Communications
Volume	57
Issue number	97
DOIs	https://doi.org/10.1039/d1cc04962c
State	Published - 18 Dec 2021

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10.1039/d1cc04962c

開發可應用於時域聚焦多光子激發顯微術之新型螢光探針:分子設計、合成與鑑定
Lin, T.
1/08/21 → 31/07/23
Project: Research
產學研發中心計畫—中央大學-科思創聯合研發中心：綠色先進功能性材料開發與製程之研究(2/3)
Lii, K.
1/07/21 → 31/12/22
Project: Research
Development of Novel Three- and Four-Photon-Absorption-Based Optical Power-Limiting Materials: Molecular Design, Synthesis and Characterization
Lin, T.
1/08/20 → 31/01/22
Project: Research

Cite this

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title = "A two-photon fluorescence probe for cell membrane imaging under temporal-focusing multiphoton excitation microscopy (TFMPEM)",

abstract = "A small-sized chromophore, BTTA-2OH, manifesting favorable solubility, large two-photon excitation efficiency, and good fluorescence photostability was synthesized to label the membrane of living cells for visualizing the dynamic movement of membrane-related vesicles via a two-photon fluorescence imaging technique based on wavelength-tunable temporal-focusing multiphoton excitation microscopy.",

author = "Lee, {Wei Hsuan} and Lai, {Jian Zong} and Hsu, {Yu Hsuan} and Cheng, {Fung Yu} and Luo, {Ching Lung} and Huang, {Yung Chin} and Lin, {Tzu Chau} and Chien, {Fan Ching}",

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T1 - A two-photon fluorescence probe for cell membrane imaging under temporal-focusing multiphoton excitation microscopy (TFMPEM)

AU - Lee, Wei Hsuan

AU - Lai, Jian Zong

AU - Hsu, Yu Hsuan

AU - Cheng, Fung Yu

AU - Luo, Ching Lung

AU - Huang, Yung Chin

AU - Lin, Tzu Chau

AU - Chien, Fan Ching

PY - 2021/12/18

Y1 - 2021/12/18

N2 - A small-sized chromophore, BTTA-2OH, manifesting favorable solubility, large two-photon excitation efficiency, and good fluorescence photostability was synthesized to label the membrane of living cells for visualizing the dynamic movement of membrane-related vesicles via a two-photon fluorescence imaging technique based on wavelength-tunable temporal-focusing multiphoton excitation microscopy.

AB - A small-sized chromophore, BTTA-2OH, manifesting favorable solubility, large two-photon excitation efficiency, and good fluorescence photostability was synthesized to label the membrane of living cells for visualizing the dynamic movement of membrane-related vesicles via a two-photon fluorescence imaging technique based on wavelength-tunable temporal-focusing multiphoton excitation microscopy.

UR - http://www.scopus.com/inward/record.url?scp=85120996657&partnerID=8YFLogxK

U2 - 10.1039/d1cc04962c

DO - 10.1039/d1cc04962c

M3 - 期刊論文

C2 - 34807218

AN - SCOPUS:85120996657

SN - 1359-7345

VL - 57

SP - 13118

EP - 13121

JO - Chemical Communications

JF - Chemical Communications

IS - 97

ER -

A two-photon fluorescence probe for cell membrane imaging under temporal-focusing multiphoton excitation microscopy (TFMPEM)

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開發可應用於時域聚焦多光子激發顯微術之新型螢光探針:分子設計、合成與鑑定

產學研發中心計畫—中央大學-科思創聯合研發中心：綠色先進功能性材料開發與製程之研究(2/3)

Development of Novel Three- and Four-Photon-Absorption-Based Optical Power-Limiting Materials: Molecular Design, Synthesis and Characterization

Cite this