A set of GFP-based organelle marker lines combined with DsRed-based gateway vectors for subcellular localization study in rice (Oryza sativa L.)

Tsung Meng Wu, Ke Chun Lin, Wei Shiang Liau, Yun Yang Chao, Ling Hung Yang, Szu Yun Chen, Chung An Lu, Chwan Yang Hong

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

In the post-genomic era, many useful tools have been developed to accelerate the investigation of gene functions. Fluorescent proteins have been widely used as protein tags for studying the subcellular localization of proteins in plants. Several fluorescent organelle marker lines have been generated in dicot plants; however, useful and reliable fluorescent organelle marker lines are lacking in the monocot model rice. Here, we developed eight different GFP-based organelle markers in transgenic rice and created a set of DsRed-based gateway vectors for combining with the marker lines. Two mitochondrial-localized rice ascorbate peroxidase genes fused to DsRed and successfully co-localized with mitochondrial-targeted marker lines verified the practical use of this system. The co-localization of GFP-fusion marker lines and DsRed-fusion proteins provide a convenient platform for in vivo or in vitro analysis of subcellular localization of rice proteins.

Original languageEnglish
Pages (from-to)107-115
Number of pages9
JournalPlant Molecular Biology
Volume90
Issue number1-2
DOIs
StatePublished - 1 Jan 2016

Keywords

  • DsRed
  • GFP
  • Organelle marker lines
  • Oryza sativa
  • Protoplast
  • Subcellular localization

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