A prokaryote and human tRNA synthetase provide an essential RNA splicing function in yeast mitochondria

Fariba Houman; Seung Bae Rho; Jiansu Zhang; Xiaoyu Shen; Chien Chia Wang; Paul Schimmel; Susan A. Martinis

doi:10.1073/pnas.240465597

A prokaryote and human tRNA synthetase provide an essential RNA splicing function in yeast mitochondria

Fariba Houman, Seung Bae Rho, Jiansu Zhang, Xiaoyu Shen, Chien Chia Wang, Paul Schimmel, Susan A. Martinis

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Mitochondrial leucyl-tRNA synthetase (LeuRS) in the yeast Saccharomyces cerevisiae provides two essential functions. In addition to aminoacylation, LeuRS functions in RNA splicing. The details of how it came to act in splicing are not known. Here we show that Mycobacterium tuberculosis and human mitochondrial LeuRSs can substitute in splicing for the S. cerevisiae mitochondrial LeuRS. Mutations of yeast mitochondrial LeuRS that had previously been shown to abolish splicing activity also eliminate splicing by the M. tuberculosis enzyme. These results suggest the role of LeuRS in splicing in yeast mitochondria results from features of the enzyme that are broadly conserved in evolution. These features are not likely to be designed for splicing per se, but instead have been adopted in yeast for that purpose.

Original language	English
Pages (from-to)	13743-13748
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	97
Issue number	25
DOIs	https://doi.org/10.1073/pnas.240465597
State	Published - 5 Dec 2000

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abstract = "Mitochondrial leucyl-tRNA synthetase (LeuRS) in the yeast Saccharomyces cerevisiae provides two essential functions. In addition to aminoacylation, LeuRS functions in RNA splicing. The details of how it came to act in splicing are not known. Here we show that Mycobacterium tuberculosis and human mitochondrial LeuRSs can substitute in splicing for the S. cerevisiae mitochondrial LeuRS. Mutations of yeast mitochondrial LeuRS that had previously been shown to abolish splicing activity also eliminate splicing by the M. tuberculosis enzyme. These results suggest the role of LeuRS in splicing in yeast mitochondria results from features of the enzyme that are broadly conserved in evolution. These features are not likely to be designed for splicing per se, but instead have been adopted in yeast for that purpose.",

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AU - Houman, Fariba

AU - Rho, Seung Bae

AU - Zhang, Jiansu

AU - Shen, Xiaoyu

AU - Wang, Chien Chia

AU - Schimmel, Paul

AU - Martinis, Susan A.

PY - 2000/12/5

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AB - Mitochondrial leucyl-tRNA synthetase (LeuRS) in the yeast Saccharomyces cerevisiae provides two essential functions. In addition to aminoacylation, LeuRS functions in RNA splicing. The details of how it came to act in splicing are not known. Here we show that Mycobacterium tuberculosis and human mitochondrial LeuRSs can substitute in splicing for the S. cerevisiae mitochondrial LeuRS. Mutations of yeast mitochondrial LeuRS that had previously been shown to abolish splicing activity also eliminate splicing by the M. tuberculosis enzyme. These results suggest the role of LeuRS in splicing in yeast mitochondria results from features of the enzyme that are broadly conserved in evolution. These features are not likely to be designed for splicing per se, but instead have been adopted in yeast for that purpose.

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A prokaryote and human tRNA synthetase provide an essential RNA splicing function in yeast mitochondria

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