A prediction of mRNA polyadenylation sites in human genes

Jorng Tzong Horng, Li Ching Wu, Shun Kai Liu, Cheng Wei Chang, Tsung Ming Chao, Rong Hwei Yeh, Kuang Fu Cheng

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

mRNA polyadenylation is an essential mechanism in human genes and is direct linked to the termination of transcription. Alternative polyadenylation changes the length of the mature mRNA's 3′UTR. Since 3′UTRs have been shown to contain regulatory elements that control mRNA functioning, alternative polyadenylation plays an important role in controlling the expression of human genes. Prediction of polyadenylation sites can help with the identification of genes and aid our understanding of the mechanisms of alternative polyadenylation. In this study, we constructed a system for mRNA polyadenylation site prediction in human genes using SVM and based on an analysis of the sequence alignment between pair-end diTags (PET) and genome sequences. The PET sequences were mapped to the reference genome more accurate compared to earlier methods. We also analyzed single-site type and multiple-site type sequences PET sequence datasets and found that the frequencies of each nucleotide were different when the single-site type and multiple-site type PET sequences were compared.

Original languageEnglish
Title of host publicationProceedings - 2011 11th IEEE International Conference on Bioinformatics and Bioengineering, BIBE 2011
Pages27-32
Number of pages6
DOIs
StatePublished - 2011
Event2011 11th IEEE International Conference on Bioinformatics and Bioengineering, BIBE 2011 - Taichung, Taiwan
Duration: 24 Oct 201126 Oct 2011

Publication series

NameProceedings - 2011 11th IEEE International Conference on Bioinformatics and Bioengineering, BIBE 2011

Conference

Conference2011 11th IEEE International Conference on Bioinformatics and Bioengineering, BIBE 2011
Country/TerritoryTaiwan
CityTaichung
Period24/10/1126/10/11

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