A novel application of indolicidin for gene delivery

Wei Wen Hu; Ze Wei Lin; Ruoh Chyu Ruaan; Wen Yih Chen; Shiow Lian Catherine Jin; Yung Chang

doi:10.1016/j.ijpharm.2013.08.035

A novel application of indolicidin for gene delivery

Wei Wen Hu, Ze Wei Lin, Ruoh Chyu Ruaan, Wen Yih Chen, Shiow Lian Catherine Jin, Yung Chang

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

A bovine derived antimicrobial peptide, indolicidin (IL), was studied of its new application for gene transfer. Plasmid DNA was complexed with both IL and polyethylenimine (PEI) as ternary particles. Compared to DNA/IL complexes, the DNA/IL/PEI particles demonstrated high zeta potentials, small particle sizes, and superior loading efficiencies, suggesting the incorporation of polycations can support IL for gene delivery. For in vitro experiments, these ternary particles significantly improved gene transfection efficiencies over the sole administrations of IL or PEI. This synergistic effect revealed that IL and PEI may play different roles for gene transfer. Our results suggest that IL should be a potential carrier for gene delivery. As our knowledge, our study should be the first article indicating the carrier ability of IL for gene transfer.

Original language	English
Pages (from-to)	293-300
Number of pages	8
Journal	International Journal of Pharmaceutics
Volume	456
Issue number	2
DOIs	https://doi.org/10.1016/j.ijpharm.2013.08.035
State	Published - 2013

Keywords

Cell-penetrating peptide
Gene delivery
Indolicidin
Nonviral vectors
Transfection

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10.1016/j.ijpharm.2013.08.035

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title = "A novel application of indolicidin for gene delivery",

abstract = "A bovine derived antimicrobial peptide, indolicidin (IL), was studied of its new application for gene transfer. Plasmid DNA was complexed with both IL and polyethylenimine (PEI) as ternary particles. Compared to DNA/IL complexes, the DNA/IL/PEI particles demonstrated high zeta potentials, small particle sizes, and superior loading efficiencies, suggesting the incorporation of polycations can support IL for gene delivery. For in vitro experiments, these ternary particles significantly improved gene transfection efficiencies over the sole administrations of IL or PEI. This synergistic effect revealed that IL and PEI may play different roles for gene transfer. Our results suggest that IL should be a potential carrier for gene delivery. As our knowledge, our study should be the first article indicating the carrier ability of IL for gene transfer.",

keywords = "Cell-penetrating peptide, Gene delivery, Indolicidin, Nonviral vectors, Transfection",

author = "Hu, {Wei Wen} and Lin, {Ze Wei} and Ruaan, {Ruoh Chyu} and Chen, {Wen Yih} and Jin, {Shiow Lian Catherine} and Yung Chang",

note = "Funding Information: This research was supported by the grant of NSC 98-2218-E-008-009 from the National Science Council of Taiwan . ",

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doi = "10.1016/j.ijpharm.2013.08.035",

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volume = "456",

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T1 - A novel application of indolicidin for gene delivery

AU - Hu, Wei Wen

AU - Lin, Ze Wei

AU - Ruaan, Ruoh Chyu

AU - Chen, Wen Yih

AU - Jin, Shiow Lian Catherine

AU - Chang, Yung

N1 - Funding Information: This research was supported by the grant of NSC 98-2218-E-008-009 from the National Science Council of Taiwan .

PY - 2013

Y1 - 2013

N2 - A bovine derived antimicrobial peptide, indolicidin (IL), was studied of its new application for gene transfer. Plasmid DNA was complexed with both IL and polyethylenimine (PEI) as ternary particles. Compared to DNA/IL complexes, the DNA/IL/PEI particles demonstrated high zeta potentials, small particle sizes, and superior loading efficiencies, suggesting the incorporation of polycations can support IL for gene delivery. For in vitro experiments, these ternary particles significantly improved gene transfection efficiencies over the sole administrations of IL or PEI. This synergistic effect revealed that IL and PEI may play different roles for gene transfer. Our results suggest that IL should be a potential carrier for gene delivery. As our knowledge, our study should be the first article indicating the carrier ability of IL for gene transfer.

AB - A bovine derived antimicrobial peptide, indolicidin (IL), was studied of its new application for gene transfer. Plasmid DNA was complexed with both IL and polyethylenimine (PEI) as ternary particles. Compared to DNA/IL complexes, the DNA/IL/PEI particles demonstrated high zeta potentials, small particle sizes, and superior loading efficiencies, suggesting the incorporation of polycations can support IL for gene delivery. For in vitro experiments, these ternary particles significantly improved gene transfection efficiencies over the sole administrations of IL or PEI. This synergistic effect revealed that IL and PEI may play different roles for gene transfer. Our results suggest that IL should be a potential carrier for gene delivery. As our knowledge, our study should be the first article indicating the carrier ability of IL for gene transfer.

KW - Cell-penetrating peptide

KW - Gene delivery

KW - Indolicidin

KW - Nonviral vectors

KW - Transfection

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U2 - 10.1016/j.ijpharm.2013.08.035

DO - 10.1016/j.ijpharm.2013.08.035

M3 - 期刊論文

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SN - 0378-5173

VL - 456

SP - 293

EP - 300

JO - International Journal of Pharmaceutics

JF - International Journal of Pharmaceutics

IS - 2

ER -

A novel application of indolicidin for gene delivery

Abstract

Keywords

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