A novel application of indolicidin for gene delivery

Wei Wen Hu, Ze Wei Lin, Ruoh Chyu Ruaan, Wen Yih Chen, Shiow Lian Catherine Jin, Yung Chang

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


A bovine derived antimicrobial peptide, indolicidin (IL), was studied of its new application for gene transfer. Plasmid DNA was complexed with both IL and polyethylenimine (PEI) as ternary particles. Compared to DNA/IL complexes, the DNA/IL/PEI particles demonstrated high zeta potentials, small particle sizes, and superior loading efficiencies, suggesting the incorporation of polycations can support IL for gene delivery. For in vitro experiments, these ternary particles significantly improved gene transfection efficiencies over the sole administrations of IL or PEI. This synergistic effect revealed that IL and PEI may play different roles for gene transfer. Our results suggest that IL should be a potential carrier for gene delivery. As our knowledge, our study should be the first article indicating the carrier ability of IL for gene transfer.

Original languageEnglish
Pages (from-to)293-300
Number of pages8
JournalInternational Journal of Pharmaceutics
Issue number2
StatePublished - 2013


  • Cell-penetrating peptide
  • Gene delivery
  • Indolicidin
  • Nonviral vectors
  • Transfection


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