A nanofluidic preconcentrator integrated with an aluminum-based nanoplasmonic sensor for Epstein-Barr virus detection

Han Yun Hsieh, Jia Xian Luo, Yi Hong Shen, Shu Cheng Lo, Yi Chiung Hsu, Hidetoshi Tahara, Yu Jui Fan, Pei Kuen Wei, Horn Jiunn Sheen

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Epstein-Barr virus (EBV) positivity is one of the indexes for diagnosing nasopharyngeal carcinoma (NPC). Moreover, systemic inflammatory responses can easily be triggered in patients who are both EBV- and coronavirus disease 2019 (COVID-19)-positive. Development of rapid and highly sensitive EBV screening methods has become important. In this study, a nanofluidic preconcentrator integrated with a nanoslit Fano resonance biosensor was developed to detect latent membrane protein 1 (LMP1) for an EBV diagnosis. Through nanoimprinting and aluminum deposition, the low-cost nanoslit plasmonic sensing chip can be mass-produced. The nanoporous membrane was patterned on a sensing chip as an ion selective channel to concentrate LMP1 proteins. Anti-LMP1 immunoglobulin G was then modified to a sensing chip to immunosense LMP1. The Fano resonant spectrum of the capped nanoslit array produced a transmission peak followed by a dip. We recorded and analyzed the spectrum using four methods, including area, center of mass, peak value, and dip value methods. With preconcentration, a limit of detection (LOD) of 100 pg/ml and a sensing range of 100 pg/ml to 10 µg/ml was achieved using the peak value.

Original languageEnglish
Article number131327
JournalSensors and Actuators, B: Chemical
Volume355
DOIs
StatePublished - 15 Mar 2022

Keywords

  • Epstein-Barr virus (EBV)
  • Latent membrane protein 1
  • Localized surface plasmon resonance
  • Nanofluidic preconcentration

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