A guided mode resonance aptasensor for thrombin detection

Sheng Fu Lin; Ting Jou Ding; Jen Tsai Liu; Chien Chieh Lee; Tsung Hsun Yang; Wen Yih Chen; Jenq Yang Chang

doi:10.3390/s110908953

A guided mode resonance aptasensor for thrombin detection

Sheng Fu Lin, Ting Jou Ding, Jen Tsai Liu, Chien Chieh Lee, Tsung Hsun Yang, Wen Yih Chen, Jenq Yang Chang

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Recent developments in aptamers have led to their widespread use in analytical and diagnostic applications, particularly for biosensing. Previous studies have combined aptamers as ligands with various sensors for numerous applications. However, merging the aptamer developments with guided mode resonance (GMR) devices has not been attempted. This study reports an aptasensor based home built GMR device. The 29-mer thrombin aptamer was immobilized on the surface of a GMR device as a recognizing ligand for thrombin detection. The sensitivity reported in this first trial study is 0.04 nm/μM for thrombin detection in the concentration range from 0.25 to 1 μM and the limit of detection (LOD) is 0.19 μM. Furthermore, the binding affinity constant (K_a) measured is in the range of 10⁶ M^-1. The investigation has demonstrated that such a GMR aptasensor has the required sensitivity for the real time, label-free, in situ detection of thrombin and provides kinetic information related to the binding.

Original language	English
Pages (from-to)	8953-8965
Number of pages	13
Journal	Sensors (Switzerland)
Volume	11
Issue number	9
DOIs	https://doi.org/10.3390/s110908953
State	Published - Sep 2011

Keywords

Affinity constant
Aptamer
Guided mode resonance
Optical biosensor
Thrombin

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title = "A guided mode resonance aptasensor for thrombin detection",

abstract = "Recent developments in aptamers have led to their widespread use in analytical and diagnostic applications, particularly for biosensing. Previous studies have combined aptamers as ligands with various sensors for numerous applications. However, merging the aptamer developments with guided mode resonance (GMR) devices has not been attempted. This study reports an aptasensor based home built GMR device. The 29-mer thrombin aptamer was immobilized on the surface of a GMR device as a recognizing ligand for thrombin detection. The sensitivity reported in this first trial study is 0.04 nm/μM for thrombin detection in the concentration range from 0.25 to 1 μM and the limit of detection (LOD) is 0.19 μM. Furthermore, the binding affinity constant (Ka) measured is in the range of 106 M-1. The investigation has demonstrated that such a GMR aptasensor has the required sensitivity for the real time, label-free, in situ detection of thrombin and provides kinetic information related to the binding.",

keywords = "Affinity constant, Aptamer, Guided mode resonance, Optical biosensor, Thrombin",

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T1 - A guided mode resonance aptasensor for thrombin detection

AU - Lin, Sheng Fu

AU - Ding, Ting Jou

AU - Liu, Jen Tsai

AU - Lee, Chien Chieh

AU - Yang, Tsung Hsun

AU - Chen, Wen Yih

AU - Chang, Jenq Yang

PY - 2011/9

Y1 - 2011/9

N2 - Recent developments in aptamers have led to their widespread use in analytical and diagnostic applications, particularly for biosensing. Previous studies have combined aptamers as ligands with various sensors for numerous applications. However, merging the aptamer developments with guided mode resonance (GMR) devices has not been attempted. This study reports an aptasensor based home built GMR device. The 29-mer thrombin aptamer was immobilized on the surface of a GMR device as a recognizing ligand for thrombin detection. The sensitivity reported in this first trial study is 0.04 nm/μM for thrombin detection in the concentration range from 0.25 to 1 μM and the limit of detection (LOD) is 0.19 μM. Furthermore, the binding affinity constant (Ka) measured is in the range of 106 M-1. The investigation has demonstrated that such a GMR aptasensor has the required sensitivity for the real time, label-free, in situ detection of thrombin and provides kinetic information related to the binding.

AB - Recent developments in aptamers have led to their widespread use in analytical and diagnostic applications, particularly for biosensing. Previous studies have combined aptamers as ligands with various sensors for numerous applications. However, merging the aptamer developments with guided mode resonance (GMR) devices has not been attempted. This study reports an aptasensor based home built GMR device. The 29-mer thrombin aptamer was immobilized on the surface of a GMR device as a recognizing ligand for thrombin detection. The sensitivity reported in this first trial study is 0.04 nm/μM for thrombin detection in the concentration range from 0.25 to 1 μM and the limit of detection (LOD) is 0.19 μM. Furthermore, the binding affinity constant (Ka) measured is in the range of 106 M-1. The investigation has demonstrated that such a GMR aptasensor has the required sensitivity for the real time, label-free, in situ detection of thrombin and provides kinetic information related to the binding.

KW - Affinity constant

KW - Aptamer

KW - Guided mode resonance

KW - Optical biosensor

KW - Thrombin

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JO - Sensors (Switzerland)

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ER -

A guided mode resonance aptasensor for thrombin detection

Abstract

Keywords

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