@inproceedings{3dd3c8e50f1d4987849c31e9a9e8c3ba,
title = "A full-field heterodyne surface plasmon resonance dynamic bio-imaging system",
abstract = "Two-dimensional (2D) phase imaging system based on phase-shift interferometry (PSI) techniques can achieve a very high accuracy, but it has a degraded dynamic characteristic due to the inherent limitation of the PSI. Hence, the phase imaging system is incapable of obtaining real-time information pertaining to phase variations. To develop a label-free, high sensitivity, and dynamic bio-imaging system, a surface plasmon resonance (SPR) biosensing is combined with full-field heterodyne interferometry to develop a common-path full-field heterodyne SPR dynamic phase imager. The phase imager provides some advantages for biosensing such as label-free sensing, high sensitivity, high throughput, long-term stability, and dynamic capability. We build a 16×16 pixel photodiode array with a frame rate of up to 10 kHz as the 2D detector as opposed to a CCD camera with 30 Hz and employ an electro-optic modulator to generate a heterodyne light source. The multi-channel and real-time demodulation is calculated by utilizing a home-made digital signal processing-based lock-in amplifier. The SPR phase imager can detect refractive index changes better than 10-6 by testing the difference between nitrogen and argon gases, and will be used to analyze the biomolecular interaction on sensing surface with high throughput screening.",
keywords = "Dynamic bio-imaging, Full-field, Heterodyne interferometry, Surface plasmon resonance",
author = "Lin, {C. H.} and Su, {Y. D.} and Huang, {C. H.} and Cho, {K. C.} and Chen, {S. J.}",
year = "2007",
doi = "10.1117/12.701749",
language = "???core.languages.en_GB???",
isbn = "0819465607",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
booktitle = "Nanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV",
note = "Nanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV ; Conference date: 23-01-2007 Through 24-01-2007",
}