A compact computational model for cell construct development in perfusion culture

C. A. Chung, C. P. Chen, T. H. Lin, C. S. Tseng

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

A problem nowadays tissue engineers encounter in developing sizable tissue implants is the nonuniform spread of cells and/or extracellular matrices. Research shows such a nutrients transport restriction may be improved by employing hydrodynamic culture systems. We propose a compact model for the simulation of cell growth in a porous construct under direct perfusion. Unlike the previous model proposed in the literature, which composes a cellular scaffold sandwiched between two culture media layers, the current model includes only the scaffold layer to simplify the mathematical and computational complex. Results show the present single-layer model can predict cell spreads and the nutrient and metabolic waste distribution as accurately as does the three-layer model. Only if the hydrodynamic aspects such as the pressure and viscous stress are prominent to know, should the more sophisticated analyses with the three-layer model be employed. The compact model provides comparable investigations for the tissue-engineering construct developments.

Original languageEnglish
Pages (from-to)1535-1541
Number of pages7
JournalBiotechnology and Bioengineering
Volume99
Issue number6
DOIs
StatePublished - 15 Apr 2008

Keywords

  • Cell culture
  • Mass transfer
  • Perfusion
  • Simulation
  • Tissue engineering

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