A common-path phase-shift interferometry surface plasmon resonance imaging system

Y. T. Su, S. J. Chen, T. L. Yeh

Research output: Contribution to journalConference articlepeer-review

2 Scopus citations


A biosensing imaging system is proposed based on the integration of surface plasmon resonance (SPR) and common-path phase-shift interferometry (PSI) techniques to measure the two-dimensional spatial phase variation caused by biomolecular interactions upon a sensing chip. The SPR phase imaging system can offer high resolution and high-throughout screening capabilities to analyze microarray biomolecular interaction without the need for additional labeling. With the long-term stability advantage of the common-path PSI technique even with external disturbances such as mechanical vibration, buffer flow noise, and laser unstable issue, the system can match the demand of real-time kinetic study for biomolecular interaction analysis (BIA). The SPR-PSI imaging system has achieved a detection limit of 2×10-7 refraction index change, a long-term phase stability of 2.5×10-4μ rms over four hours, and a spatial phase resolution of 10-3 π with a lateral resolution of 100μm.

Original languageEnglish
Article number26
Pages (from-to)144-151
Number of pages8
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
StatePublished - 2005
EventPlasmonics in Biology and Medicine II - San Jose, CA, United States
Duration: 24 Jan 200525 Jan 2005


  • DNA microarray
  • Imaging system
  • Phase-shifting interferometry
  • Surface plasmon resonance


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