A Bienzyme Sensor for the Determination of Hypoxanthine and Inosine

Shengshui Hu; Chung Chiun Liu

doi:10.1002/elan.1140091510

A Bienzyme Sensor for the Determination of Hypoxanthine and Inosine

Shengshui Hu, Chung Chiun Liu

Graduate Institute of Materials Science and Engineering

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

A biosensor for the detection of hypoxanthine (Hx) and inosine (HxR) has been developed by the coimmobilization of nucleoside phosphorylase (NP) and xanthine oxidase (XO) on a chemically modified electrode, which uses a glassy carbon electrode coated with a Nafion - paraquat film. This sensor responds to Hx and HxR in the presence of phosphate, and it responds only to Hx in the absence of phosphate (borate buffer as medium). The sensor measurement is based on the oxygen consumed due to the enzymatic reaction involving xanthine oxidase which is immobilized on the electrode surface. In this study, the experimental parameters are optimized and the linear relationships between the decreased currents and the concentrations of both Hx and HxR in the range of 1 × 10^-6-2 × 10^-4 M are obtained. This biosensor exhibits a high degree of sensitivity and can be used for the assay for Hx and HxR in fish meat or other biological samples.

Original language	English
Pages (from-to)	1174-1179
Number of pages	6
Journal	Electroanalysis
Volume	9
Issue number	15
DOIs	https://doi.org/10.1002/elan.1140091510
State	Published - Oct 1997

Keywords

Biosensor
Enzyme electrode
Hypoxanthine
Inosine
Nucleoside phosphorylase
Xanthine oxidase

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10.1002/elan.1140091510

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title = "A Bienzyme Sensor for the Determination of Hypoxanthine and Inosine",

abstract = "A biosensor for the detection of hypoxanthine (Hx) and inosine (HxR) has been developed by the coimmobilization of nucleoside phosphorylase (NP) and xanthine oxidase (XO) on a chemically modified electrode, which uses a glassy carbon electrode coated with a Nafion - paraquat film. This sensor responds to Hx and HxR in the presence of phosphate, and it responds only to Hx in the absence of phosphate (borate buffer as medium). The sensor measurement is based on the oxygen consumed due to the enzymatic reaction involving xanthine oxidase which is immobilized on the electrode surface. In this study, the experimental parameters are optimized and the linear relationships between the decreased currents and the concentrations of both Hx and HxR in the range of 1 × 10-6-2 × 10-4 M are obtained. This biosensor exhibits a high degree of sensitivity and can be used for the assay for Hx and HxR in fish meat or other biological samples.",

keywords = "Biosensor, Enzyme electrode, Hypoxanthine, Inosine, Nucleoside phosphorylase, Xanthine oxidase",

author = "Shengshui Hu and Liu, {Chung Chiun}",

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T1 - A Bienzyme Sensor for the Determination of Hypoxanthine and Inosine

AU - Hu, Shengshui

AU - Liu, Chung Chiun

PY - 1997/10

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N2 - A biosensor for the detection of hypoxanthine (Hx) and inosine (HxR) has been developed by the coimmobilization of nucleoside phosphorylase (NP) and xanthine oxidase (XO) on a chemically modified electrode, which uses a glassy carbon electrode coated with a Nafion - paraquat film. This sensor responds to Hx and HxR in the presence of phosphate, and it responds only to Hx in the absence of phosphate (borate buffer as medium). The sensor measurement is based on the oxygen consumed due to the enzymatic reaction involving xanthine oxidase which is immobilized on the electrode surface. In this study, the experimental parameters are optimized and the linear relationships between the decreased currents and the concentrations of both Hx and HxR in the range of 1 × 10-6-2 × 10-4 M are obtained. This biosensor exhibits a high degree of sensitivity and can be used for the assay for Hx and HxR in fish meat or other biological samples.

AB - A biosensor for the detection of hypoxanthine (Hx) and inosine (HxR) has been developed by the coimmobilization of nucleoside phosphorylase (NP) and xanthine oxidase (XO) on a chemically modified electrode, which uses a glassy carbon electrode coated with a Nafion - paraquat film. This sensor responds to Hx and HxR in the presence of phosphate, and it responds only to Hx in the absence of phosphate (borate buffer as medium). The sensor measurement is based on the oxygen consumed due to the enzymatic reaction involving xanthine oxidase which is immobilized on the electrode surface. In this study, the experimental parameters are optimized and the linear relationships between the decreased currents and the concentrations of both Hx and HxR in the range of 1 × 10-6-2 × 10-4 M are obtained. This biosensor exhibits a high degree of sensitivity and can be used for the assay for Hx and HxR in fish meat or other biological samples.

KW - Biosensor

KW - Enzyme electrode

KW - Hypoxanthine

KW - Inosine

KW - Nucleoside phosphorylase

KW - Xanthine oxidase

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U2 - 10.1002/elan.1140091510

DO - 10.1002/elan.1140091510

M3 - 期刊論文

AN - SCOPUS:0345911522

VL - 9

SP - 1174

EP - 1179

JO - Electroanalysis

JF - Electroanalysis

SN - 1040-0397

IS - 15

ER -

A Bienzyme Sensor for the Determination of Hypoxanthine and Inosine

Abstract

Keywords

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