A Bienzyme Sensor for the Determination of Hypoxanthine and Inosine

Shengshui Hu, Chung Chiun Liu

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

A biosensor for the detection of hypoxanthine (Hx) and inosine (HxR) has been developed by the coimmobilization of nucleoside phosphorylase (NP) and xanthine oxidase (XO) on a chemically modified electrode, which uses a glassy carbon electrode coated with a Nafion - paraquat film. This sensor responds to Hx and HxR in the presence of phosphate, and it responds only to Hx in the absence of phosphate (borate buffer as medium). The sensor measurement is based on the oxygen consumed due to the enzymatic reaction involving xanthine oxidase which is immobilized on the electrode surface. In this study, the experimental parameters are optimized and the linear relationships between the decreased currents and the concentrations of both Hx and HxR in the range of 1 × 10-6-2 × 10-4 M are obtained. This biosensor exhibits a high degree of sensitivity and can be used for the assay for Hx and HxR in fish meat or other biological samples.

Original languageEnglish
Pages (from-to)1174-1179
Number of pages6
JournalElectroanalysis
Volume9
Issue number15
DOIs
StatePublished - Oct 1997

Keywords

  • Biosensor
  • Enzyme electrode
  • Hypoxanthine
  • Inosine
  • Nucleoside phosphorylase
  • Xanthine oxidase

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