454 - Electrochemical studies of metal ion inhibition of polyacrylamide gel immobilized glucose oxidase

C. C. Liu; F. M. Fryburg; A. K. Chen

doi:10.1016/0302-4598(81)87012-2

454 - Electrochemical studies of metal ion inhibition of polyacrylamide gel immobilized glucose oxidase

C. C. Liu, F. M. Fryburg, A. K. Chen

Graduate Institute of Materials Science and Engineering

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Metal ion inhibitions of polyacrylamide gel immobilized glucose oxidase were investigated. Both Hg²⁺ and Ag⁺ ions were the subjects of this study, in the oxidation of β-D-glucose catalyzed by immobilized glucose oxidase. Electric potential measurement was used as a means to determine the inhibition effect. Experimental results show that the inhibition effects of Hg²⁺ and Ag⁺ ions on immobilized glucose oxidase were similar to those observed in free enzyme system. Reversibility of the immobilized enzyme matrix for metal ion inhibition study is reasonably good for a period of 48 hours. The detection level of the metal ions presented in the medium by this electrochemical method is, however, limited.

Original language	English
Pages (from-to)	703-708
Number of pages	6
Journal	Bioelectrochemistry and Bioenergetics
Volume	8
Issue number	6
DOIs	https://doi.org/10.1016/0302-4598(81)87012-2
State	Published - Dec 1981

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title = "454 - Electrochemical studies of metal ion inhibition of polyacrylamide gel immobilized glucose oxidase",

abstract = "Metal ion inhibitions of polyacrylamide gel immobilized glucose oxidase were investigated. Both Hg2+ and Ag+ ions were the subjects of this study, in the oxidation of β-D-glucose catalyzed by immobilized glucose oxidase. Electric potential measurement was used as a means to determine the inhibition effect. Experimental results show that the inhibition effects of Hg2+ and Ag+ ions on immobilized glucose oxidase were similar to those observed in free enzyme system. Reversibility of the immobilized enzyme matrix for metal ion inhibition study is reasonably good for a period of 48 hours. The detection level of the metal ions presented in the medium by this electrochemical method is, however, limited.",

author = "Liu, {C. C.} and Fryburg, {F. M.} and Chen, {A. K.}",

note = "Funding Information: This work was partially supported by Lewis Research Center, NASA, under grant No. NSG 3203. Advice provided by Dr. J. Stuart Fordyce of NASA is gratefully appreciated.",

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T1 - 454 - Electrochemical studies of metal ion inhibition of polyacrylamide gel immobilized glucose oxidase

AU - Liu, C. C.

AU - Fryburg, F. M.

AU - Chen, A. K.

N1 - Funding Information: This work was partially supported by Lewis Research Center, NASA, under grant No. NSG 3203. Advice provided by Dr. J. Stuart Fordyce of NASA is gratefully appreciated.

PY - 1981/12

Y1 - 1981/12

N2 - Metal ion inhibitions of polyacrylamide gel immobilized glucose oxidase were investigated. Both Hg2+ and Ag+ ions were the subjects of this study, in the oxidation of β-D-glucose catalyzed by immobilized glucose oxidase. Electric potential measurement was used as a means to determine the inhibition effect. Experimental results show that the inhibition effects of Hg2+ and Ag+ ions on immobilized glucose oxidase were similar to those observed in free enzyme system. Reversibility of the immobilized enzyme matrix for metal ion inhibition study is reasonably good for a period of 48 hours. The detection level of the metal ions presented in the medium by this electrochemical method is, however, limited.

AB - Metal ion inhibitions of polyacrylamide gel immobilized glucose oxidase were investigated. Both Hg2+ and Ag+ ions were the subjects of this study, in the oxidation of β-D-glucose catalyzed by immobilized glucose oxidase. Electric potential measurement was used as a means to determine the inhibition effect. Experimental results show that the inhibition effects of Hg2+ and Ag+ ions on immobilized glucose oxidase were similar to those observed in free enzyme system. Reversibility of the immobilized enzyme matrix for metal ion inhibition study is reasonably good for a period of 48 hours. The detection level of the metal ions presented in the medium by this electrochemical method is, however, limited.

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U2 - 10.1016/0302-4598(81)87012-2

DO - 10.1016/0302-4598(81)87012-2

M3 - 期刊論文

AN - SCOPUS:0005265537

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SP - 703

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JO - Bioelectrochemistry and Bioenergetics

JF - Bioelectrochemistry and Bioenergetics

SN - 0302-4598

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ER -

454 - Electrochemical studies of metal ion inhibition of polyacrylamide gel immobilized glucose oxidase

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