4-Aminobiphenyl suppresses homologous recombination repair by a reactive oxygen species-dependent p53/miR-513a-5p/p53 loop

Heng Dao Lin, Chao Ling Yao, Wei Jen Ou, Yueh Hsia Luo, Ssu Ching Chen

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

4-Aminobiphenyl (4-ABP), a well-known human carcinogen, can cause oxidative DNA damage and induce miR-513a-5p. However, the interplay between miR-513a-5p and DNA damage remains unclear. In our result of ChIP assay, we speculated that p53 as transcription factor could regulate miR-513a-5p expression. In addition, we found that miR-513a-5p-induced by 4-ABP could suppress p53 expression and HR repair activity. On the other hand, the levels of p53, miR-513a-5p, and γH2AX were attenuated by 5 mM N-acetyl-L-cysteine (NAC) pretreatment, indicating that the reactive oxygen species (ROS)-dependent p53-miR-513a-5p was involved in DSB repair in 4-ABP-treated cells. These findings indicated that the ROS/p53/miR-513a-5p/p53 loop axis plays a relevant role in regulating HR repair which may facilitate our understanding of molecular mechanisms regarding how miR-513a-5p impacts DSB repair in 4-ABP-treated cells.

Original languageEnglish
Article number152580
JournalToxicology
Volume444
DOIs
StatePublished - Nov 2020

Keywords

  • 4-Aminobiphenyl
  • DNA homologous recombination repair
  • miR-513a-5p
  • p53
  • ROS

Fingerprint

Dive into the research topics of '4-Aminobiphenyl suppresses homologous recombination repair by a reactive oxygen species-dependent p53/miR-513a-5p/p53 loop'. Together they form a unique fingerprint.

Cite this