3D porous calcium-alginate scaffolds cell culture system improved human osteoblast cell clusters for cell therapy

Ching Yun Chen, Cherng Jyh Ke, Ko Chung Yen, Hui Chen Hsieh, Jui Sheng Sun, Feng Huei Lin

Research output: Contribution to journalArticlepeer-review

62 Scopus citations

Abstract

Age-related orthopedic disorders and bone defects have become a critical public health issue, and cell-based therapy is potentially a novel solution for issues surrounding bone tissue engineering and regenerative medicine. Long-term cultures of primary bone cells exhibit phenotypic and functional degeneration; therefore, culturing cells or tissues suitable for clinical use remain a challenge. A platform consisting of human osteoblasts (hOBs), calcium-alginate (Ca-Alginate) scaffolds, and a self-made bioreactor system was established for autologous transplantation of human osteoblast cell clusters. The Ca-Alginate scaffold facilitated the growth and differentiation of human bone cell clusters, and the functionally-closed process bioreactor system supplied the soluble nutrients and osteogenic signals required to maintain the cell viability. This system preserved the proliferative ability of cells and cell viability and up-regulated bone-related gene expression and biological apatite crystals formation. The bone-like tissue generated could be extracted by removal of calcium ions via ethylenediaminetetraacetic acid (EDTA) chelation, and exhibited a size suitable for injection. The described strategy could be used in therapeutic application and opens new avenues for surgical interventions to correct skeletal defects.

Original languageEnglish
Pages (from-to)643-655
Number of pages13
JournalTheranostics
Volume5
Issue number6
DOIs
StatePublished - 2015

Keywords

  • Autografts
  • Bone-like tissues
  • Perfusion
  • Preformed scaffolds
  • Three-dimensional culture

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