262 - Quantitation of glucose concentration using a glucose oxidase-catalase electrode by potentiometric measurement

C. C. Liu, L. B. Wingard, S. K. Wolfson, S. J. Yao, A. L. Drash, J. G. Schiller

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Glucose oxidase and catalase were immobilized in polyacrylamide gel around a platinum screen and used to measure the concentration of glucose in 0.1 M phosphate buffer, pH 7.3, at 37°C. This enzyme electrode produced a direct potentiometric signal with reference to a standard Ag|AgCl electrode. The potential difference was linearly related to the logarithm of the concentration of glucose over the range of 3-40 cg/dm3 (mg/dl). The electrode was stable for at least 32 hours at 37°C. Hydrogen peroxide, generated by the oxidation of glucose and depleted by decomposition and diffusion, was the apparent source of the potentiometric signal. Direct potentiometric measurement has the advantages of simple readout, continuous detection, and small electrode size, and does not require external polarising potential. This approach should be suitable for the continuous in vivo monitoring of blood glucose levels if the upper limit of specificity for glucose (presently 40 mg/dl) can be extended.

Original languageEnglish
Pages (from-to)19-26
Number of pages8
JournalBioelectrochemistry and Bioenergetics
Volume6
Issue number1
DOIs
StatePublished - Mar 1979

Fingerprint

Dive into the research topics of '262 - Quantitation of glucose concentration using a glucose oxidase-catalase electrode by potentiometric measurement'. Together they form a unique fingerprint.

Cite this