The Integrated Analysis of Transcriptome and Proteome for Exploring the Biodegradation Mechanism of 2, 4, 6-Trinitrotoluene by Citrobacter Sp.(3/3)

Project Details

Description

Soil and groundwater contaminated by munitions compounds has been a crucial issue in environmental protection. Trinitrotoluene (TNT) has been classified as carcinogens. The aim of this proposal is to evaluate the potential of Citrobacter sp. applied to biodegrade TNT-contaminated soil/water. A total of consecutive three years will be focused on bioremediation as follows. First year, we will establish the optimal conditions for bioremediation by Citrobacter sp., including the maximal tolerance of TNT toxicity by this bacterium, the byproducts of TNT bioconversion, and the application of bioreactor containing contaminated soil/water mixed with Citrobacter sp. Second year, the whole genome of Citrobacter sp.will be established as the reference template due to its lacking genome. Using Citrobacter sp. as the mapping reference, we compared the transcriptome responses in between Citrobacter sp.with and without TNT, which deduce the molecular mechanisms of TNT biodegradation by Citrobacter sp. Additionally, some differential genes related to TNT bioremediation/resistance will be validated by qRT-PCR. Third year, we will perform the proteomic analysis for Citrobacter sp. to biodegrade TNT or not. The combined analysis of proteomic and transcriptome will enable us to find out the key enzyme for bioremediation. After the purification of the enzyme, we determine its specificity, activity at the defined pH, and kinetic studies. Finally, the immobilized enzyme will be evaluated for its potency in enhancing TNT bioremediation.
StatusFinished
Effective start/end date1/08/2031/07/21

UN Sustainable Development Goals

In 2015, UN member states agreed to 17 global Sustainable Development Goals (SDGs) to end poverty, protect the planet and ensure prosperity for all. This project contributes towards the following SDG(s):

  • SDG 11 - Sustainable Cities and Communities
  • SDG 15 - Life on Land
  • SDG 17 - Partnerships for the Goals

Keywords

  • Trinitrotoluene
  • Citrobacter sp.
  • Proteomic
  • Transcriptome
  • Bioremediation

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