Developments of 3d Paper Based Nucelic Acid Extraction Kit for Point-Of-Care Liquid Biopsy(2/3)

This proposal is based on researches outcomes and knowledge built from the MOST undergoing project “Studies of binding mechanism between nucleic acids with silicasurface of liquid biopsy and its applications in biomolecular diagnosis”. We are proposing to develop a 3D paper based nucleic acid, especially for miRNA, extraction kit for low-resource settings disease/cancer screening. Paper based analytical diagnostics (PAD) has attracted extensive attention due to its low cast, simple fabrication and can be coupled with complex microfluidic function. However, attentions are mostly in diagnostics and for mostly protein/enzyme detection. The high hurdle of purification (sample preparation) of nucleic acids for the paper diagnostics limits and poorly suited for the applications of the PAD in nucleic acids detection. In this project, we propose a 3D paper based miRNA extraction kit. The aim is to develop a purification/extraction kit of miRNA from cell and liquid tissues. We will incorporate exosome purification, protein precipitation, nucleic acids extraction and miRNA enhancement and recovery into the 3D PAD. Antibody for exosome, solvent design and silica powder adsorption are the basic mechanism applied for the 3D fluidic design.