Metal-organic frameworks (MOFs) has emerged as a novel type of crystalline porousmaterial that combines highly desirable properties of zeolites, such as crystallinity,microporosity, high surface area, and exceptional thermal and chemical stability.Emerging functional applications of MOFs in gas separation, catalysis, and sensinghave been recently reported. However, so far, MOFs few studies have been reported inbioapplications, especially in fields of enzymatic catalysis/bioreactor and enzymaticbiosensors.In order to continuously expend our previous MOST project, in this project, wepropose many novel experiments for furtherly study on MOFs materials in enzymaticapplications. Basically, we based on our recent two publications: "ImpartingFunctionality to Biocatalysts via Embedding Enzymes into Nanoporous Materials by ade Novo Approach: Size-Selective Sheltering of Catalase in Metal−Organic FrameworkMicrocrystals" J. Am. Chem. Soc. (2015), 137, 4276-4279 and “Water-Based Synthesisof Zeolitic Imidazolate Framework-90 (ZIF-90) with a Controllable Particle Size”(Chem Eur. J 34 (2013) 11139 –11142). It opens an alternative avenue for theapplicability of ZIFs, a subclass of MOFs, in enzyme catalysis and bioreactor. ZIFsobtained by water synthetic system would be used as a novel platform for theencapsulation of enzyme or biochemical for further enzymatic catalysis, bioreactor orbiosensor and etc. Therefore, our major aims in this project are focusing on developingwater-based synthetic systems for obtaining MOFs as to platforms for embeddingenzymes for enzymatic catalysts, biosensors or bioreactors.Herein, we demonstrate a new concept that accomplishes to not only enhance thestability and recyclability but also impart new functions to the enzyme compositesthrough use of a de novo approach to embed enzymes into MOFs supports of pore sizessmaller than the size of the enzyme. The de novo approach allows MOFs with poresizes smaller than the size of the enzymes to be used. This not only prevents leachingbut also greatly expands the selection of enzymes and MOFs, making the methodgenerally applicable for various functional applications. It offers a novel tool toimmobilize and impart new functions to biomolecules such as proteins, DNA, and RNA.This study, we try to develop more MOFs, i.e., UiO-66, MIL-101 or Zr-Fumarate,which can be obtained under water-based and milder condition in order to combineenzymes such as HRP, Luciferase, Glucose Oxidase or Invertase and etc. Thosecomposites of Enzyme@MOFs can be applied in several fields including biomoleculardelivery, size-selective enzyme catalysis, industrial wastewater treatment, bioreactorand etc.