Develop high-efficiency dechlorination consortia and optimize the bioaugmentation strategy of bioremediation in chlorinated contamination: pilot study

Organic halide compounds such as trichloroethyene are refractory pollutants, which often pollute the groundwater in our country and are difficult to remediate. The decommissioning rate of polluted sites is only less than 20%. At present, the biggest bottleneck encountered in remediation is that operators often use chemical agents for biological remediation. Remediation eventually leads to the accumulation of vinyl chloride, which is more toxic and carcinogenic. This factor often makes the remediation of a site fail. Through cooperation with the industry in the past, our team has collected groundwater contaminated by chlorinated organic compounds throughout Taiwan, and further cultivated Taiwan's native dechlorination bacteria. Using our unique Dehalococcoides culture platform, we have successfully found several consortia. These consortia can quickly and less produce secondary pollution such as vinyl chloride, to develop innovative novel bioaugmentation strategy. In the first year of this project, through the laboratory of the anaerobic dechlorination bacteria database established by the on-site model field, we will develop the dechlorination consortia used in the field. We will use the fermentation tank in this project to develop. The production process enables the consortia to reach the standard that can be applied to on-site remediation, and at the same time establishes the degradation efficiency of the dechlorination bacteria agent. In the second year, this research will enter the field test in the field, optimize the active bio-enhancement method, use the double packer injection method to improve the influence range and accuracy of the inoculum infusion, find the best parameters and procedures for the implementation of the construction method, and effectively improve the inoculum's performance. use. The use of consortia agent can maximize the efficacy of biological substrates, reduce the cost of using biological substrates and shorten the remediation time. At the same time, consortia agent can effectively solve the problem of vinyl chloride accumulation. It is expected that the use of dechlorination consortia bioaugmentation method can effectively improve remediation bottleneck of organic halide contamination in Taiwan.