Our previous results have shown that a rice small heat shock protein (sHSP) gene, OsHSP16.9A, was activated under heat stress (HS) and expressed during seed maturation. To further study the physiological function of OsHSP16.9A, we established Oshsp16.9A-overexpression and -underexpression transgenic rice plants, Oshsp16.9A-OE and Oshsp16.9A-RNAi, respectively. In addition, we also developed the transgenic rice plants transformed with Oshsp18.0 (Oshsp18.0-OE), which shares 74% identity (84% similarity) in protein sequence with Oshsp16.9A, as the control. Phenotype analysis revealed that the growth was retarded and heading time was delayed in Oshsp16.9A-RNAi plants compared with the wild-type (WT), Oshsp16.9A-OE, and Oshsp18.0-OE plants. The grains of 16.9A-OEs have more densely packed and irregularly polyhedral starch granules than those of the WT, Oshsp16.9A-RNAis, and Oshsp18.0-OEs. Thermotolerance analysis indicated that 16.9A-OEs showed a significantly increase in seed survivals after heat treatment, whereas WT, Oshsp16.9A-RNAi, and Oshsp18.0-OE seeds were not able to endure HS-induced detrimental effects. In addition, overexpression of Oshsp16.9A can improve the survival rates of seedlings and pollen grains of transgenic rice plants exposed to HS. Furthermore, using microarray and proteomics analysis, we found that Oshsp16.9A directly or indirectly interacted with Oshsp101 to function in seed thermotolerance and upregulated some starch synthesis enzymes in seeds under HS. In this three-year project, our major goals are to further study the role of Oshsp16.9A in seed thermotolerance and reduction of chalkiness. Firstly, we are trying to investigate the interaction between Oshsp16.9A and Oshsp101 in rice seeds. These results will help us to understand the physiological function of Oshsp16.9A and Oshsp101 in rice seeds. Secondly, we plan to characterize the expression patterns of starch synthesis enzymes in Oshsp16.9A-overexpressing rice seeds and test whether Oshsp16.9A can function in the mutants of these starch synthesis enzymes. Finally, we try to compare expression level of Oshsp16.9A in the thermoresistant and thermosensitive rice varieties. Based on these results, we are able to establish Oshsp16.9A as a screening marker for thermotolerant rice.
|Effective start/end date||1/08/20 → 31/07/21|
UN Sustainable Development Goals
In 2015, UN member states agreed to 17 global Sustainable Development Goals (SDGs) to end poverty, protect the planet and ensure prosperity for all. This project contributes towards the following SDG(s):
- small heat shock protein
- heat stress
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