n the past three years, I investigated several areas of fundamental physical principles in various areas of biophysics, nonlinear physics, and non-equilibrium statistical physics. Many of these projects would involve analytic techniques, such as nonlinear dynamical analysis and statistical physics techniques, and often accompanied by numerical methods. Most of these have close connections with relevant experiments. All these problems are of current research interest and are important to the fundamental understanding of the basic physics in these bio and complex systems as well as in potential applications. The results are very fruitful, leading to 25 papers published in the leading journals in the field of statistical physics, biophysics, and complex systems: 1 in Physical Review Letters, 1 in Nature Communications, 6 in Physical Review Research, 14 in Physical Review E, 1 in Nonlinear Dynamics, and 2 in Chinese Journal of Physics.