雷射誘導石墨烯電極於細胞基底行為電阻抗感測

Project Details

Description

Laser-induced Graphene (LIG) electrodes will be fabricated for cellular behavior monitoring with the Electric Cell-substrate Impedance Sensing (ECIS) method. The graphene electrodes can be formed by applying laser beams on the surface of polyimide substrate. The high intrinsic electron mobility and porous structure of the graphene electrodes will lead to high electrical conductivity and thus high sensitivity. The polyimide substrate is suitable for cell measurement, considering its flexibility and biocompatibility. Interdigitated LIG electrodes of different geometries will be fabricated and tested to choose the most suitable geometrical parameters. The performance of the LIG electrodes will be validated by Electrochemical Impedance Spectroscopy (EIS). Fibroblast L929 cells will be cultured in nutrient medium wherein the fabricated LIG electrodes are situated. The quantity of cell attachment/detachment on the electrode surfaces affects the impedance between electrodes. Hence, the measured impedance spectrum across the electrodes reflects the cell behaviors on the electrodes. The developed ECIS method with LIG electrodes will be used to assess the cells’ reaction to cytotoxic agents. The success of the project goal could lead to significant contribution in life science, biomedicine and clinical diagnostics. Through this research, we hope to advance the technology toward the clinical applications in the fields of diagnosis, healthcare, and pharmaceutical researches.
StatusFinished
Effective start/end date1/08/2231/10/23

UN Sustainable Development Goals

In 2015, UN member states agreed to 17 global Sustainable Development Goals (SDGs) to end poverty, protect the planet and ensure prosperity for all. This project contributes towards the following SDG(s):

  • SDG 9 - Industry, Innovation, and Infrastructure
  • SDG 17 - Partnerships for the Goals

Keywords

  • Electric Cell-substrate Impedance Sensing
  • laser-induced graphene
  • interdigital electrode
  • impedance biosensor
  • cell behavior
  • polyimide

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