The autonomic nervous system plays a crucial regulatory role in human health. Dysregulation of the autonomic nervous system can lead to various diseases. In recent studies, the application of pulsed electromagnetic field (PEMF) therapy has garnered significant attention for balancing the autonomic nervous system (ANS). PEMF therapy does not require direct contact with the user's body and is a non-invasive, non-contact, and non-thermal treatment that does not cause damage to the skin even with long-term use. However, current PEMF devices tend to be bulky due to the large size of the magnetic coil required to generate sufficient magnetic strength for stimulation. This study proposes a multi-layer PEMF device manufactured using flexible printed circuit board (FPCB). The stimulation coil in the proposed system offers advantages of being bendable, thin, lightweight, and producing high magnetic field intensity. The PEMF system has been applied to regulate ANS balance during posture-induced sympathetic/parasympathetic changes in tilt-table tests. The PEMF stimulation has shown significant improvement in regulating ANS balance. Comparing the PEMF condition with the non-PEMF condition, the spectral HRV indices LF, HF, and LF/HF ratio were 62.02±19.35 vs. 71.17±14.34, 37.98±19.35 vs. 28.83±14.34, and 2.62±2.41 vs. 4.03±3.96, respectively, indicating the effectiveness of PEMF stimulation in ANS regulation. The proposed wearable PEMF device offers good wearability and can be integrated with clothing, enabling long-term stimulation in future human studies.