深共熔溶劑作為新興綠色化學溶劑的製備與其在分析化學應用之研究(3/3)

In this three-year proposal, we will focus on (i) preparation of deep eutecticsolvents (DESs) as green extracting solvents for microextraction; (ii) applicationof DESs for the extracting solvents of various emerging contaminants in aquaticand urine samples; (iii) application of DESs to extract various emergingcontaminants in indoor dust, biota and vegetable/fruits samples. Deep eutecticsolvents, a group of novel “green” solvents, formed by mixing of variousquaternary ammonium salts (i.e., choline chloride) and different hydrogen-bonddonors (HBD, such as urea, amines or alcohols). They are easily synthesized atroom temperature, and no need purification afterward. Therefore, DESs havebecome attractive alternative solvents for various scientific research, andsubstituted conventional volatile organic solvents, especially for hazardouschlorinated solvents. In this proposal, we will focus on preparing variousproperties DESs (i.e., hydrophilicity or lipophilicity) to extract different emergingcontaminants from various environmental matrices, biota and food samples. Tominimize the number of experiments, costs, and reagents required, optimizationof the parameters affecting microextraction sample pretreatment procedures willbe done via multivariate experimental designs. The state-of-the-art UHPLCHRMS will be developed and evaluated for the identification and quantitation ofthe target analytes at trace-level. Followings four major Tasks are going to beinvestigated and studied:Task 1. Deep eutectic solvents preparation, characterization and selection toextract various emerging contaminants (i.e., Microcystins, parabenspreservatives, high production volume chemicals (HPVs): Benzotriazoles andBenzothiazoles, as the model compounds) in various sample matrices;Task 2. For liquid samples, evaluating DES-based vortex-assisted liquid-liquidmicroextraction (VA-LLME), ultrasound-assisted liquid-liquid microextraction (UALLME). For solid samples, such as indoor dust, biota samples, andvegetables/fruits samples, evaluating modified matrix solid-phase dispersion (MSPD) and modified QuEChERS procedures;Task 3. Optimizing the main parameters affecting microextraction procedures byvarious multivariate experimental designs (i.e., response surface design coupledwith Box-Behnken Design, Plackett-Burman design or central composite design);Task 4. Evaluating the influence of DES in final extract, and for the first time, toevaluate the feasibility of the UHPLC-ESI-HRMS for the identification andquantitation of the target analytes at trace-level.The tasks studied in this proposal will significantly contribute to wide spectrum ofDES-based applications in sample pretreatment and microextraction techniques,and also developed the state-of-the-art hyphenated mass spectrometric todetermine various emerging contaminants in various environmental and biotasamples at trace-level. Eventually, we hope many advantages of DES-basedmicroextraction procedures could fulfill the “green analytical chemistry”guidelines, and could greatly aid future routine analysis and monitoring programson the occurrence of selected emerging contaminants. Moreover, the results ofthis research should be highly in response to our environmental protection trends,promote food safety and public health concern, as well as conservation andpollution control policies in Taiwan.