多功能瞬時干涉顯微技術之研發:定量相位分析方法與在活體生物和生物感測上之應用(2/3)

  • Li, Meng-Chi (PI)

Project Details

Description

A multifunctional snapshot interferometric microscope platform is proposed toachieve the real-time measurement for living cells reconstruction and a novelmultiplex biosensing technology. A polarization CMOS camera with a linearmicro-polarizer array accomplishes snapshot phase-shifting measurement andcan obtain single-shot measurement of quantitative birefringence and phaseimage information. In the study, the 2 ambiguities removed by the definition of afringe order or using multi-wavelengths to extend the vertical measurementrange. To achieve the high measurement accuracy, in addition to ensure thequality of the optical components, the error correction algorithm is critical as well.A simple and effective algorithm which Lissajous ellipse fitting algorithm will beaddressed the issues caused by the inaccurate phase shift. We believe thatsnapshot interferometric microscope platform in the study will be a useful tool inmeasuring quantitative phase information of live cells. Therefore, we expect thatthe dynamic quantitative phase image will not only show high sensitivity, highspecificity and reproducible capability but also offer a high-throughput andmultiplex method of a biosensing technology.
StatusFinished
Effective start/end date1/08/2231/07/23

UN Sustainable Development Goals

In 2015, UN member states agreed to 17 global Sustainable Development Goals (SDGs) to end poverty, protect the planet and ensure prosperity for all. This project contributes towards the following SDG(s):

  • SDG 11 - Sustainable Cities and Communities
  • SDG 17 - Partnerships for the Goals

Keywords

  • Dynamic interferometry、Snapshot quantitative phase images、Interferometric microscope、living cell structure、biosensor、Multiplexed detection

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