In order to cope with the increasing drought frequency as the consequence of global warming, the development of drought-resistance cultivars becomes more and more important for maintaining the competitive tea plantation and related industry in Taiwan. The molecular mechanism related to drought-resistance (DR) phenotypes would be themajor source for developing molecular markers to facilitate the selection of the desired traits during such processes. In this study, the differential gene expressions in response to water deficiency from var. sinenesis (small-leaf variety) cultivars, TTES No.12 (high DR) and Chin-Shin-Oolong (low DR) which are widely planted in Taiwan, will be compared with those from TTES No. 19 (low DR) and TTES no. 22 (high DR), which are hybrids of previous two cultivars. By using the RNA sequencing technology, both the genes involved in drought resistance and DNA diversity associated with drought tolerance are anticipated to be revealed for further application in cultivar development.The tea plants of TTES No. 19 and TTES no. 22 were acquired from the seedling nurseries with the plant variety rights of these two cultivars, while the tea plants of TTES No.12 and Chin-Shin-Oolong were acquired according to the availability in a seedling nursery. The one- to three years-old tea plants were transplanted and cultured in black polypropylene pots in Tea Research and Extension Station, COA in Yangmei, Taoyuan. During the active growth phase in the late summer and early autumn, the tea plants of each cultivar were randomly assigned to eight groups and subjected to control, drought-stress, and drought stress- recovery treatments. The soil water contents, pot weight, plant water potential at turgor loss point were measured along with the collection of shoot cuttings in 1-4 days intervals. The total RNA samples, including 3 replicates per treatment per cultivar, from the buds, were extracted and sent to NGS company for RNA-seq using Illumina platform. Meanwhile, we also collected the bud samples from the tea plantation on the TRES campus to test the RNA extraction and qRT-PCR protocol earlier this year. The PCR reactions were carried out to test nine pairs of primers and the amplification efficiencies of three primers for reference genes were also investigated by the standard curve analysis.As this research project is executed, we intend to achieve three anticipated objects. First, the resource for better research activities in the Department of Life Sciences and Tea Research and Extension Station can be integrated and the long-term research collaborations between the two parties were established. Second, the sequence and expression levels of genes in four small-leaf cultivars, which exhibit high economic impact in Taiwan tea industry, will be acquired and provided for future research projects. Last and most important, the molecular mechanisms of drought resistance in the small-leaf variety that are identified in this study can be used as molecular markers and further applied in the development of drought-resistance cultivars of var. sinensis .
|Effective start/end date||1/02/21 → 31/12/21|
UN Sustainable Development Goals
In 2015, UN member states agreed to 17 global Sustainable Development Goals (SDGs) to end poverty, protect the planet and ensure prosperity for all. This project contributes towards the following SDG(s):
- Camellia sinesis
- Molecular mechanism of drought resistance
- Comparative transcriptomic analysis
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